Dr. Alexey V. Fedulov has an MD in Internal Medicine and a PhD in Immunology/Allergy and Pathophysiology from St. Petersburg Pavlov Medical University. He completed his postdoctoral fellowship at Harvard School of Public Health and later became Assistant Professor at Harvard Medical School / Brigham and Women's Hospital. His research has been awarded with a K99/R00 Pathway to Independence Award from NIH. He has developed a program of independently funded experimental studies, trained fellows and students and taught Pathophysiology of Human Disease course for Harvard students in the Public Health programs.
He joined Brown and the RIH community in 2018 to expand his studies in epigenetics.
| Aghagoli G, Fedulov AV, Shinnick JK. "Epigenetics and recurrent urinary tract infections: an update on current research and potential clinical application." Epigenomics, vol. 15, no. 19, 2023, pp. 961-964. |
| Broadwin M, Aghagoli G, Sabe SA, Harris DD, Wallace J, Lawson J, Ragayendran A, Fedulov AV, Sellke FW. "Extracellular vesicle treatment partially reverts epigenetic alterations in chronically ischemic porcine myocardium." Vessel plus, vol. 7, 2023. |
| Ramar M, Yano N, Fedulov AV. "Intra-Airway Treatment with Synthetic Lipoxin A4 and Resolvin E2 Mitigates Neonatal Asthma Triggered by Maternal Exposure to Environmental Particles." International Journal of Molecular Sciences, vol. 24, no. 7, 2023. |
| Al-Shuweli S, Landt E, Ellervik C, Poulsen HE, Ramar M, Dahl M, Fedulov AV. "Risk of asthma in offspring of asthmatic fathers versus mothers: A population-based study of 21,000 individuals in Denmark." Respiratory Medicine, vol. 207, 2023, pp. 107116. |
| Yano N, Fedulov AV. "Targeted DNA Demethylation: Vectors, Effectors and Perspectives." Biomedicines, vol. 11, no. 5, 2023. |
| Wiscovitch-Russo, Rosana, Singh, Harinder, Oldfield, Lauren M., Fedulov, Alexey V., Gonzalez-Juarbe, Norberto. "An optimized approach for processing of frozen lung and lavage samples for microbiome studies." PLOS ONE, vol. 17, no. 4, 2022, pp. e0265891. |
| Tuazon JA, Kilburg-Basnyat B, Oldfield LM, Wiscovitch-Russo R, Dunigan-Russell K, Fedulov AV, Oestreich KJ, Gowdy KM. "Emerging Insights into the Impact of Air Pollution on Immune-Mediated Asthma Pathogenesis." Current Allergy and Asthma Reports, vol. 22, no. 7, 2022, pp. 77-92. |
| Kumar M, Yano N, Fedulov AV. "Gestational exposure to titanium dioxide, diesel exhaust, and concentrated urban air particles affects levels of specialized pro-resolving mediators in response to allergen in asthma-susceptible neonate lungs." Journal of toxicology and environmental health. Part A, vol. 85, no. 6, 2022, pp. 243-261. |
| Wiscovitch-Russo R, Taal AM, Kuelbs C, Oldfield LM, Ramar M, Singh H, Fedulov AV, Gonzalez-Juarbe N. "Gut and lung microbiome profiles in pregnant mice." Frontiers in Microbiology, vol. 13, 2022, pp. 946779. |
| Wheatley LM, Holloway JW, Svanes C, Sears MR, Breton C, Fedulov AV, Nilsson E, Vercelli D, Zhang H, Togias A, Arshad SH. "The role of epigenetics in multi-generational transmission of asthma: An NIAID workshop report-based narrative review." Clinical & Experimental Allergy, vol. 52, no. 11, 2022, pp. 1264-1275. |
| Yano N, Emi T, Gregory DJ, Fedulov AV. "Consideration on Efficient Recombinant Protein Production: Focus on Substrate Protein-Specific Compatibility Patterns of Molecular Chaperones." The protein journal, vol. 40, no. 5, 2021, pp. 756-764. |
| Aghagoli G, Del Re A, Yano N, Zhang Z, Gheit AA, Phillips RK, Sellke FW, Fedulov AV. "Methylome of skeletal muscle tissue in patients with hypertension and diabetes undergoing cardiopulmonary bypass." Epigenomics, vol. 13, no. 23, 2021, pp. 1853-1866. |
| Emi T, Rivera LM, Tripathi VC, Yano N, Ragavendran A, Wallace J, Fedulov AV. "Transcriptomic and epigenomic effects of insoluble particles on J774 macrophages." Epigenetics, vol. 16, no. 10, 2021, pp. 1053-1070. |
| Mandarino A, Gregory DJ, McGuire CC, Leblanc BW, Witt H, Rivera LM, Godleski JJ, Fedulov AV. "The effect of talc particles on phagocytes in co-culture with ovarian cancer cells." Environmental Research, vol. 180, 2020, pp. 108676. |
| Campion A, Smith KJ, Fedulov AV, Gregory DZ, Fan Y, Godleski JJ. "Identification of Foreign Particles in Human Tissues Using Raman Microscopy." Analytical Chemistry, vol. 90, no. 14, 2018, pp. 8362-8369. |
| Gregory DJ, Kobzik L, Yang Z, McGuire CC, Fedulov AV. "Transgenerational transmission of asthma risk after exposure to environmental particles during pregnancy." American Journal of Physiology-Lung Cellular and Molecular Physiology, vol. 313, no. 2, 2017, pp. L395-L405. |
| Zhang Y, Mikhaylova L, Kobzik L, Fedulov AV. "Estrogen-mediated impairment of macrophageal uptake of environmental TiO2 particles to explain inflammatory effect of TiO2 on airways during pregnancy." Journal of immunotoxicology, vol. 12, no. 1, 2015, pp. 81-91. |
| Lim R, Fedulov AV, Kobzik L. "Maternal stress during pregnancy increases neonatal allergy susceptibility: role of glucocorticoids." American Journal of Physiology-Lung Cellular and Molecular Physiology, vol. 307, no. 2, 2014, pp. L141-8. |
| Hu R, Pan W, Fedulov AV, Jester W, Jones MR, Weiss ST, Panettieri RA Jr, Tantisira K, Lu Q. "MicroRNA-10a controls airway smooth muscle cell proliferation via direct targeting of the PI3 kinase pathway." The FASEB Journal, vol. 28, no. 5, 2014, pp. 2347-57. |
| Ysasi AB, Belle JM, Gibney BC, Fedulov AV, Wagner W, AkiraTsuda, Konerding MA, Mentzer SJ. "Effect of unilateral diaphragmatic paralysis on postpneumonectomy lung growth." American Journal of Physiology-Lung Cellular and Molecular Physiology, vol. 305, no. 6, 2013, pp. L439-45. |
| Mikhaylova L, Zhang Y, Kobzik L, Fedulov AV. "Link between epigenomic alterations and genome-wide aberrant transcriptional response to allergen in dendritic cells conveying maternal asthma risk." PLoS ONE, vol. 8, no. 8, 2013, pp. e70387. |
| Gregory DJ, Zhang Y, Kobzik L, Fedulov AV. "Specific transcriptional enhancement of inducible nitric oxide synthase by targeted promoter demethylation." Epigenetics, vol. 8, no. 11, 2013, pp. 1205-12. |
| Williams AS, Kasahara DI, Verbout NG, Fedulov AV, Zhu M, Si H, Wurmbrand AP, Hug C, Ranscht B, Shore SA. "Role of the adiponectin binding protein, T-cadherin (Cdh13), in allergic airways responses in mice." PLoS ONE, vol. 7, no. 7, 2012, pp. e41088. |
| Gregory DJ, Mikhaylova L, Fedulov AV. "Selective DNA demethylation by fusion of TDG with a sequence-specific DNA-binding domain." Epigenetics, vol. 7, no. 4, 2012, pp. 344-9. |
| Fedulov AV, Kobzik L. "Allergy risk is mediated by dendritic cells with congenital epigenetic changes." American Journal of Respiratory Cell and Molecular Biology, vol. 44, no. 3, 2011, pp. 285-92. |
| Zhu M, Liu PY, Kasahara DI, Williams AS, Verbout NG, Halayko AJ, Fedulov A, Shoji T, Williams ES, Noma K, Shore SA, Liao JK. "Role of Rho kinase isoforms in murine allergic airway responses." European Respiratory Journal, vol. 38, no. 4, 2011, pp. 841-50. |
| Lamoureux DP, Kobzik L, Fedulov AV. "Customized PCR-array analysis informed by gene-chip microarray and biological hypothesis reveals pathways involved in lung inflammatory response to titanium dioxide in pregnancy." Journal of toxicology and environmental health. Part A, vol. 73, no. 9, 2010, pp. 596-606. |
| Fedulov AV, Kobzik L. "Immunotoxicologic analysis of maternal transmission of asthma risk." Journal of immunotoxicology, vol. 5, no. 4, 2008, pp. 445-52. |
| Fedulov AV, Leme A, Yang Z, Dahl M, Lim R, Mariani TJ, Kobzik L. "Pulmonary exposure to particles during pregnancy causes increased neonatal asthma susceptibility." American Journal of Respiratory Cell and Molecular Biology, vol. 38, no. 1, 2008, pp. 57-67. |
| Sigaud S, Goldsmith CA, Zhou H, Yang Z, Fedulov A, Imrich A, Kobzik L. "Air pollution particles diminish bacterial clearance in the primed lungs of mice." Toxicology and Applied Pharmacology, vol. 223, no. 1, 2007, pp. 1-9. |
| Fedulov AV, Leme AS, Kobzik L. "Duration of allergic susceptibility in maternal transmission of asthma risk." American Journal of Reproductive Immunology, vol. 58, no. 2, 2007, pp. 120-8. |
| Lim RH, Arredouani MS, Fedulov A, Kobzik L, Hubeau C. "Maternal allergic contact dermatitis causes increased asthma risk in offspring." Respiratory Research, vol. 8, 2007, pp. 56. |
| Arredouani MS, Franco F, Imrich A, Fedulov A, Lu X, Perkins D, Soininen R, Tryggvason K, Shapiro SD, Kobzik L. "Scavenger Receptors SR-AI/II and MARCO limit pulmonary dendritic cell migration and allergic airway inflammation." The Journal of Immunology, vol. 178, no. 9, 2007, pp. 5912-20. |
| Fedulov A, Silverman E, Xiang Y, Leme A, Kobzik L. "Immunostimulatory CpG oligonucleotides abrogate allergic susceptibility in a murine model of maternal asthma transmission." The Journal of Immunology, vol. 175, no. 7, 2005, pp. 4292-300. |
| Fedulov AV, Ses TP, Gavrisheva NA, Rybakova MG, Vassilyeva JG, Tkachenko SB, Kallner A, MacMillan JC. "Serum TGF-beta 1 and TNF-alpha levels and cardiac fibrosis in experimental chronic renal failure." Immunological investigations, vol. 34, no. 2, 2005, pp. 143-52. |
| Emel'ianov AV, Zinakova MK, Krasnoshchekova OI, Sinitsina TM, Rudinskiĭ KA, Fedulov AV. "[Quality of life and characteristics of the external respiration function in patients with bronchial asthma]." Terapevticheskii arkhiv, vol. 73, no. 12, 2001, pp. 63-5. |
| Emelyanov A, Fedoseev G, Abulimity A, Rudinski K, Fedoulov A, Karabanov A, Barnes PJ. "Elevated concentrations of exhaled hydrogen peroxide in asthmatic patients." Chest, vol. 120, no. 4, 2001, pp. 1136-9. |
| Emel'ianov AV, Shcherbak IG, Abulimiti A, Krasnoshchekova OI, Sinitsyna TM, Rudinskiĭ KA, Fedulov AV, Karabanov AV. "[Hydrogen peroxide--a marker for respiratory tract inflammation in patients with bronchial asthma]." Terapevticheskii arkhiv, vol. 72, no. 12, 2000, pp. 27-30. |
Fedulov laboratory studies epigenetic regulation and develops molecular tools for epigenetic engineering.
Any cell in an organism includes all genes of that organism's genome. The difference in structure and function of cells results from epigenetic silencing of 'unnecessary genes' which occurs in great part by DNA methylation. Taking control of the epigenome is a novel and exciting biomedical challenge. Moreover, in disease the aberrant DNA methylation in key regulatory areas (e.g. promoters) can inhibit gene transcription. There is an increasing wealth of DNA methylation abnormalities in numerous pathologies, however we usually cannot say if these abnormalities are in fact causative for the disease. Another major challenge is that no effective approach currently exists to gene-specifically demethylate a target area, and little is known about DNA demethylation mechanisms.
We develop custom-designed molecules of putative DNA demethylases fused with sequence-specific DNA binding domains and use these tools to re-activate or enhance the silenced intrinsic genes in cells. The method will be broadly applicable as an experimental tool in epigenetics and may open avenues to a novel class of therapeutics.
Our models focus on inflammation, tissue repair and immune regulation with potential applications in lung disease (fibrosis, COPD, asthma) and in surgical research. We are especially pursuing epigenetic and microbiome alterations in asthma triggered by exposure to environmental toxicants like particulate matter.
| Year | Degree | Institution |
|---|---|---|
| 2003 | PhD | Saint Petersburg State Medical University |
| 2000 | MD | Saint Petersburg State Medical University |
| Postdoctoral Fellow | Harvard School of Public Health | 2003-2007 |
The Warren Alpert Medical School of Brown University.
Rhode Island Hospital. Department of Surgery, Divsion of Surgical Research.
