| Miller, Bryant D., Smail, Benedict A., Van Eeuwen, Trevor, Kodama, Hanna, Kondo, Kazuma, Jiang, Hua, O’Brien, Allison, Dai, Nan, Trachman, Robert J., Guan, Shengxi, Karlow, Jennifer A., Cheng, Wen-Chih, Sedivy, John M., Jogl, Gerwald, Rout, Michael P., LaCava, John, Burns, Kathleen H., Taylor, Martin S. "Rapid DNA cleavage by the LINE-1 endonuclease proximal to DNA ends and at mismatches." Journal of Biological Chemistry, vol. 302, no. 1, 2026, pp. 110994. |
| Belato, Helen B, Knight, Alexa L, D'Ordine, Alexandra M, Pindi, Chinmai, Fan, Zhiqiang, Luo, Jinping, Palermo, Giulia, Jogl, Gerwald, Lisi, George P. "Structural and dynamic impacts of single-atom disruptions to guide RNA interactions within the recognition lobe of Geobacillus stearothermophilus Cas9." eLife, vol. 13, 2025. |
| Skeens, Erin, Sinha, Souvik, Ahsan, Mohd, D’Ordine, Alexandra M., Jogl, Gerwald, Palermo, Giulia, Lisi, George P. "High-fidelity, hyper-accurate, and evolved mutants rewire atomic-level communication in CRISPR-Cas9." Science Advances, vol. 10, no. 10, 2024. |
| D'Ordine AM, Jogl G, Sedivy JM. "Identification and characterization of small molecule inhibitors of the LINE-1 retrotransposon endonuclease." Nature Communications, vol. 15, no. 1, 2024, pp. 3883. |
| Clark NE, Katolik A, Gallant P, Welch A, Murphy E, Buerer L, Schorl C, Naik N, Naik MT, Holloway SP, Cano K, Weintraub ST, Howard KM, Hart PJ, Jogl G, Damha M, Fairbrother WG. "Activation of human RNA lariat debranching enzyme Dbr1 by binding protein TTDN1 occurs though an intrinsically disordered C-terminal domain." Journal of Biological Chemistry, vol. 299, no. 9, 2023, pp. 105100. |
| Pindi, Chinmai, Sinha, Souvik, Belato, Helen, Norbrun, Carmelissa, Luo, Jinping, D'Ordine, Alexandra M., Jogl, Gerwald, Lisi, George, Palermo, Giulia. "Dynamics and thermostability of HNH nuclease in divergent Cas9 species." Biophysical Journal, vol. 122, no. 3, 2023, pp. 138a. |
| Belato, Helen B., Norbrun, Carmelissa, Luo, Jinping, Pindi, Chinmai, Sinha, Souvik, D’Ordine, Alexandra M., Jogl, Gerwald, Palermo, Giulia, Lisi, George P. "Disruption of electrostatic contacts in the HNH nuclease from a thermophilic Cas9 rewires allosteric motions and enhances high-temperature DNA cleavage." The Journal of Chemical Physics, vol. 157, no. 22, 2022, pp. 225103. |
| Belato, Helen B., D'Ordine, Alexandra M., Nierzwicki, Lukasz, Arantes, Pablo R., Jogl, Gerwald, Palermo, Giulia, Lisi, George P. "Structural and dynamic insights into the HNH nuclease of divergent Cas9 species." Journal of Structural Biology, vol. 214, no. 1, 2022, pp. 107814. |
| Belato, Helen, D’Ordine, Alexandra, Jogl, Gerwald, Lisi, George. "Unique structural and dynamic properties of the HNH nuclease in mesophilic and thermophilic Cas9 revealed by NMR." The FASEB Journal, vol. 36, no. S1, 2022. |
| Newton JC, Naik MT, Li GY, Murphy EL, Fawzi NL, Sedivy JM, Jogl G. "Phase separation of the LINE-1 ORF1 protein is mediated by the N-terminus and coiled-coil domain." Biophysical Journal, vol. 120, no. 11, 2021, pp. 2181-2191. |
| East KW, Newton JC, Morzan UN, Narkhede YB, Acharya A, Skeens E, Jogl G, Batista VS, Palermo G, Lisi GP. "Allosteric Motions of the CRISPR-Cas9 HNH Nuclease Probed by NMR and Molecular Dynamics." J. Am. Chem. Soc., vol. 142, no. 3, 2020, pp. 1348-1358. |
| Murphy, Eileen L., Singh, Kavindra V., Avila, Bryant, Kleffmann, Torsten, Gregory, Steven T., Murray, Barbara E., Krause, Kurt L., Khayat, Reza, Jogl, Gerwald. "Cryo-electron microscopy structure of the 70S ribosome from Enterococcus faecalis." Scientific Reports, vol. 10, no. 1, 2020, pp. 16301. |
| Killeavy, Erin E., Jogl, Gerwald, Gregory, Steven T. "Tiamulin-Resistant Mutants of the Thermophilic Bacterium Thermus thermophilus." Antibiotics, vol. 9, no. 6, 2020, pp. 313. |
| Kuzu G, Kaye EG, Chery J, Siggers T, Yang L, Dobson JR, Boor S, Bliss J, Liu W, Jogl G, Rohs R, Singh ND, Bulyk ML, Tolstorukov MY, Larschan E. "Expansion of GA Dinucleotide Repeats Increases the Density of CLAMP Binding Sites on the X-Chromosome to Promote Drosophila Dosage Compensation." PLOS Genetics, vol. 12, no. 7, 2016, pp. e1006120. |
| Duan L, Jogl G, Cane DE. "The Cytochrome P450-Catalyzed Oxidative Rearrangement in the Final Step of Pentalenolactone Biosynthesis: Substrate Structure Determines Mechanism." J. Am. Chem. Soc., vol. 138, no. 38, 2016, pp. 12678-89. |
| Carr JF, Lee HJ, Jaspers JB, Dahlberg AE, Jogl G, Gregory ST. "Phenotypic Suppression of Streptomycin Resistance by Mutations in Multiple Components of the Translation Apparatus." Journal of Bacteriology, vol. 197, no. 18, 2015, pp. 2981-8. |
| Gregory ST, Connetti JL, Carr JF, Jogl G, Dahlberg AE. "Phenotypic interactions among mutations in a Thermus thermophilus 16S rRNA gene detected with genetic selections and experimental evolution." Journal of bacteriology, vol. 196, no. 21, 2014, pp. 3776-83. |
| Demirci H, Murphy FV 4th, Murphy EL, Connetti JL, Dahlberg AE, Jogl G, Gregory ST. "Structural Analysis of Base Substitutions in Thermus thermophilus 16S rRNA Conferring Streptomycin Resistance." Antimicrobial Agents and Chemotherapy, vol. 58, no. 8, 2014, pp. 4308-17. |
| Demirci H, Murphy F 4th, Murphy E, Gregory ST, Dahlberg AE, Jogl G. "A structural basis for streptomycin-induced misreading of the genetic code." Nature Communications, vol. 4, 2013, pp. 1355. |
| Li H, Jogl G. "Crystal structure of decaprenylphosphoryl-β- D-ribose 2'-epimerase from Mycobacterium smegmatis." Proteins, vol. 81, no. 3, 2013, pp. 538-43. |
| Demirci H, Sierra RG, Laksmono H, Shoeman RL, Botha S, Barends TR, Nass K, Schlichting I, Doak RB, Gati C, Williams GJ, Boutet S, Messerschmidt M, Jogl G, Dahlberg AE, Gregory ST, Bogan MJ. "Serial femtosecond X-ray diffraction of 30S ribosomal subunit microcrystals in liquid suspension at ambient temperature using an X-ray free-electron laser." Acta crystallographica. Section F, Structural biology and crystallization communications, vol. 69, no. Pt 9, 2013, pp. 1066-9. |
| Demirci H, Wang L, Murphy FV 4th, Murphy EL, Carr JF, Blanchard SC, Jogl G, Dahlberg AE, Gregory ST. "The central role of protein S12 in organizing the structure of the decoding site of the ribosome." RNA, vol. 19, no. 12, 2013, pp. 1791-801. |
| Larsen LH, Rasmussen A, Giessing AM, Jogl G, Kirpekar F. "Identification and characterization of the Thermus thermophilus 5-methylcytidine (m5C) methyltransferase modifying 23 S ribosomal RNA (rRNA) base C1942." Journal of Biological Chemistry, vol. 287, no. 33, 2012, pp. 27593-600. |
| Jogl G, Wang X, Mason SA, Kovalevsky A, Mustyakimov M, Fisher Z, Hoffman C, Kratky C, Langan P. "High-resolution neutron crystallographic studies of the hydration of the coenzyme cob(II)alamin." Acta crystallographica. Section D, Biological crystallography, vol. 67, no. Pt 6, 2011, pp. 584-91. |
| Demirci H, Murphy F 4th, Belardinelli R, Kelley AC, Ramakrishnan V, Gregory ST, Dahlberg AE, Jogl G. "Modification of 16S ribosomal RNA by the KsgA methyltransferase restructures the 30S subunit to optimize ribosome function." RNA, vol. 16, no. 12, 2010, pp. 2319-24. |
| Demirci H, Larsen LH, Hansen T, Rasmussen A, Cadambi A, Gregory ST, Kirpekar F, Jogl G. "Multi-site-specific 16S rRNA methyltransferase RsmF from Thermus thermophilus." RNA, vol. 16, no. 8, 2010, pp. 1584-96. |
| Li H, Jogl G. "Structural and biochemical studies of TIGAR (TP53-induced glycolysis and apoptosis regulator)." Journal of Biological Chemistry, vol. 284, no. 3, 2009, pp. 1748-54. |
| Gregory ST, Demirci H, Belardinelli R, Monshupanee T, Gualerzi C, Dahlberg AE, Jogl G. "Structural and functional studies of the Thermus thermophilus 16S rRNA methyltransferase RsmG." RNA, vol. 15, no. 9, 2009, pp. 1693-704. |
| Demirci H, Belardinelli R, Seri E, Gregory ST, Gualerzi C, Dahlberg AE, Jogl G. "Structural rearrangements in the active site of the Thermus thermophilus 16S rRNA methyltransferase KsgA in a binary complex with 5'-methylthioadenosine." Journal of molecular biology, vol. 388, no. 2, 2009, pp. 271-82. |
| Demirci H, Gregory ST, Dahlberg AE, Jogl G. "Crystal structure of the Thermus thermophilus 16 S rRNA methyltransferase RsmC in complex with cofactor and substrate guanosine." Journal of Biological Chemistry, vol. 283, no. 39, 2008, pp. 26548-56. |
| Demirci H, Gregory ST, Dahlberg AE, Jogl G. "Multiple-site trimethylation of ribosomal protein L11 by the PrmA methyltransferase." Structure (London, England : 1993), vol. 16, no. 7, 2008, pp. 1059-66. |
| Demirci, H., Gregory, S.T., Dahlberg, A.E., Jogl, G. "Recognition and catalysis of ribosomal protein L11 by the protein trimethyltransferase PrmA." Acta Cryst Sect A, vol. 64, no. a1, 2008, pp. C374-C374. |
| You Z, Omura S, Ikeda H, Cane DE, Jogl G. "Crystal structure of the non-heme iron dioxygenase PtlH in pentalenolactone biosynthesis." Journal of Biological Chemistry, vol. 282, no. 50, 2007, pp. 36552-60. |
| Li H, Jogl G. "Crystal structure of the zinc-binding transport protein ZnuA from Escherichia coli reveals an unexpected variation in metal coordination." Journal of molecular biology, vol. 368, no. 5, 2007, pp. 1358-66. |
| Demirci H, Gregory ST, Dahlberg AE, Jogl G. "Recognition of ribosomal protein L11 by the protein trimethyltransferase PrmA." EMBO J, vol. 26, no. 2, 2007, pp. 567-77. |
| Holmes W, Jogl G. "Crystal structure of inositol phosphate multikinase 2 and implications for substrate specificity." Journal of Biological Chemistry, vol. 281, no. 49, 2006, pp. 38109-16. |
| Hsiao YS, Jogl G, Esser V, Tong L. "Crystal structure of rat carnitine palmitoyltransferase II (CPT-II)." Biochemical and Biophysical Research Communications, vol. 346, no. 3, 2006, pp. 974-80. |
| Hsiao YS, Jogl G, Tong L. "Crystal structures of murine carnitine acetyltransferase in ternary complexes with its substrates." Journal of Biological Chemistry, vol. 281, no. 38, 2006, pp. 28480-7. |
| Jogl G, Hsiao YS, Tong L. "Crystal structure of mouse carnitine octanoyltransferase and molecular determinants of substrate selectivity." Journal of Biological Chemistry, vol. 280, no. 1, 2005, pp. 738-44. |
| Gebauer D, Li J, Jogl G, Shen Y, Myszka DG, Tong L. "Crystal structure of the PH-BEACH domains of human LRBA/BGL." Biochemistry, vol. 43, no. 47, 2004, pp. 14873-80. |
| Jogl G, Tong L. "Crystal structure of yeast acetyl-coenzyme A synthetase in complex with AMP." Biochemistry, vol. 43, no. 6, 2004, pp. 1425-31. |
| Hsiao YS, Jogl G, Tong L. "Structural and biochemical studies of the substrate selectivity of carnitine acetyltransferase." Journal of Biological Chemistry, vol. 279, no. 30, 2004, pp. 31584-9. |
| Jogl G, Hsiao YS, Tong L. "Structure and function of carnitine acyltransferases." Annals of the New York Academy of Sciences, vol. 1033, 2004, pp. 17-29. |
| Jogl G, Tong L. "Crystal structure of carnitine acetyltransferase and implications for the catalytic mechanism and fatty acid transport." Cell, vol. 112, no. 1, 2003, pp. 113-22. |
| Gobin S, Thuillier L, Jogl G, Faye A, Tong L, Chi M, Bonnefont JP, Girard J, Prip-Buus C. "Functional and structural basis of carnitine palmitoyltransferase 1A deficiency." Journal of Biological Chemistry, vol. 278, no. 50, 2003, pp. 50428-34. |
| Jogl G, Rozovsky S, McDermott AE, Tong L. "Optimal alignment for enzymatic proton transfer: structure of the Michaelis complex of triosephosphate isomerase at 1.2-A resolution." Proceedings of the National Academy of Sciences, vol. 100, no. 1, 2003, pp. 50-5. |
| Jogl G, Shen Y, Gebauer D, Li J, Wiegmann K, Kashkar H, Krönke M, Tong L. "Crystal structure of the BEACH domain reveals an unusual fold and extensive association with a novel PH domain." EMBO J, vol. 21, no. 18, 2002, pp. 4785-95. |
| Jogl G, Tao X, Xu Y, Tong L. "COMO: a program for combined molecular replacement." Acta crystallographica. Section D, Biological crystallography, vol. 57, no. Pt 8, 2001, pp. 1127-34. |
| Rozovsky S, Jogl G, Tong L, McDermott AE. "Solution-state NMR investigations of triosephosphate isomerase active site loop motion: ligand release in relation to active site loop dynamics." Journal of molecular biology, vol. 310, no. 1, 2001, pp. 271-80. |
| Champloy F, Gruber K, Jogl G, Kratky C. "XAS spectroscopy reveals X-ray-induced photoreduction of free and protein-bound B12 cofactors." Journal of synchrotron radiation, vol. 7, no. Pt 4, 2000, pp. 267-73. |
| Reitzer R, Gruber K, Jogl G, Wagner UG, Bothe H, Buckel W, Kratky C. "Glutamate mutase from Clostridium cochlearium: the structure of a coenzyme B12-dependent enzyme provides new mechanistic insights." Structure (London, England : 1993), vol. 7, no. 8, 1999, pp. 891-902. |
| Langan P, Lehmann M, Wilkinson C, Jogl G, Kratky C. "Neutron Laue diffraction studies of coenzyme cob(II)alamin." Acta crystallographica. Section D, Biological crystallography, vol. 55, no. Pt 1, 1999, pp. 51-9. |
| Reitzer R, Krasser M, Jogl G, Buckel W, Bothe H, Kratky C. "Crystallization and preliminary X-ray analysis of recombinant glutamate mutase and of the isolated component S from Clostridium cochlearium." Acta crystallographica. Section D, Biological crystallography, vol. 54, no. Pt 5, 1998, pp. 1039-42. |
Our research focuses on the structure, dynamics and biomolecular function of ribonucleoprotein complexes. We use cryo-electron microscopy and x-ray crystallography in combination with biophysical and biochemical assays to study ribosomes, retrotransposons, and the Crispr-Cas system.
The ribosome is a large molecular machine that translates the genetic code in messenger RNA (mRNA) molecules into proteins. The structure and the function of the ribosome is highly conserved in all domains of life. Despite this high conservation, however, the bacterial ribosome is the target for many antibiotic compounds used to treat human diseases. in published work, we showed that the antibiotic streptomycin induces structural rearrangements in the ribosomal decoding center that are likely to disrupt the allosteric signaling pathway active during the decoding of incoming tRNAs, leading to miscoding, the insertion of the wrong amino acid into the growing peptide chain. Related work examined the molecular basis for streptomycin resistance mutations and for the unusual streptomycin dependence mutations. Ongoing work used cryo-electron microscopy to examine ribosome dynamics during translation and how these motions are affected by antibiotic inhibitors.
In another project, we recently discovered small-molecule inhibitors against the endonuclease domain of the LINE-1 ORF2 protein and characterized their activity biochemically and in cell culture (in collaboration with Dr. John Sedivy). We also discovered that the LINE-1 ORF1 protein serves as RNA chaperone and is capable of phase separation.
A third project, in collaboration with Dr. George Lisi, focuses on the structural dynamics and allosteric regulation of CRISPR-Cas9.
NIH R01 GM094157 (9/15/10 - 8/31/26)
Role: PI (MPI with Dr. Steven Gregory (University of Rhode Island)
Structural robustness of ribosome functional centers
| Year | Degree | Institution |
|---|---|---|
| 1999 | PhD | Karl Franzens Universitat Graz |
| 1994 | MSc | Karl Franzens Universitat Graz |
| Postdoctoral Associate | Columbia University, Biology | 1999-2004 | New York, New York, USA |
2018 Elizabeth LeDuc Award for Excellence in Teaching in the Life Sciences
1994 M.Sc. Thesis Award of the Austrian Chemical Society
| Name | Title |
|---|---|
| Lisi, George | Associate Professor, Co-Director of Graduate Studies for the MCB Graduate Program |
| Sedivy, John | Hermon C. Bumpus Professor of Biology, Associate Dean and Director, Center for the Biology of Aging |
| BIOL 0280 - Biochemistry |
| BIOL 0280 - Introductory Biochemistry |
| BIOL 0285 - Inquiry in Biochemistry: From Gene to Protein Function |
| BIOL 1270 - Advanced Biochemistry |
| BIOL 2030 - Foundations for Advanced Study in the Life Sciences |
| BIOL 2270 - Advanced Biochemistry |
