https://www.brown.edu/research/labs/brossay/
https://orcid.org/0000-0002-7497-8488
| Borys SM, Reilly SP, Magill I, Zemmour D, Brossay L. "NK cells restrain cytotoxic CD8(+) T cells in the submandibular gland via PD-1-PD-L1." Science immunology, vol. 9, no. 102, 2024, pp. eadl2967. |
| Hogan MJ, Maheshwari N, Begg BE, Nicastri A, Hedgepeth EJ, Muramatsu H, Pardi N, Miller MA, Reilly SP, Brossay L, Lynch KW, Ternette N, Eisenlohr LC. "Cryptic MHC-E epitope from influenza elicits a potent cytolytic T cell response." Nature Immunology, 2023. |
| Borys SM, Bag AK, Brossay L, Adeegbe DO. "The Yin and Yang of Targeting KLRG1+ Tregs and Effector Cells." Frontiers in immunology, vol. 13, 2022, pp. 894508. |
| Tata A, Dodard G, Fugère C, Leget C, Ors M, Rossi B, Vivier E, Brossay L. "Combination blockade of KLRG1 and PD-1 promotes immune control of local and disseminated cancers." Oncoimmunology, vol. 10, no. 1, 2021, pp. 1933808. |
| Seillet C, Brossay L, Vivier E. "Natural killers or ILC1s? That is the question." Current Opinion in Immunology, vol. 68, 2021, pp. 48-53. |
| Zhang J, Le Gras S, Pouxvielh K, Faure F, Fallone L, Kern N, Moreews M, Mathieu AL, Schneider R, Marliac Q, Jung M, Berton A, Hayek S, Vidalain PO, Marçais A, Dodard G, Dejean A, Brossay L, Ghavi-Helm Y, Walzer T. "Sequential actions of EOMES and T-BET promote stepwise maturation of natural killer cells." Nature Communications, vol. 12, no. 1, 2021, pp. 5446. |
| McNamara JT, Huntington KE, Borys S, Jayasuriya CT, Brossay L. "SHP-2 deletion in CD4Cre expressing chondrocyte precursors leads to tumor development with wrist tropism." Scientific reports, vol. 11, no. 1, 2021, pp. 20006. |
| Dodard G, Tata A, Erick TK, Jaime D, Miah SMS, Quatrini L, Escalière B, Ugolini S, Vivier E, Brossay L. "Inflammation-Induced Lactate Leads to Rapid Loss of Hepatic Tissue-Resident NK Cells." Cell Reports, vol. 32, no. 1, 2020, pp. 107855. |
| Anderson CK, Reilly SP, Brossay L. "The Invariant NKT Cell Response Has Differential Signaling Requirements during Antigen-Dependent and Antigen-Independent Activation." The Journal of Immunology, vol. 206, no. 1, 2020, pp. ji2000870. |
| Anderson CK, Reilly EC, Lee AY, Brossay L. "Qa-1-Restricted CD8<sup>+</sup> T Cells Can Compensate for the Absence of Conventional T Cells during Viral Infection." Cell Reports, vol. 27, no. 2, 2019, pp. 537-548.e5. |
| Niogret C, Miah SMS, Rota G, Fonta NP, Wang H, Held W, Birchmeier W, Sexl V, Yang W, Vivier E, Ho PC, Brossay L, Guarda G. "Shp-2 is critical for ERK and metabolic engagement downstream of IL-15 receptor in NK cells." Nature Communications, vol. 10, no. 1, 2019, pp. 1444. |
| Erick T, Grigoryan L, Brossay L. "Lacrimal Gland NK Cells Are Developmentally and Functionally Similar to Conventional NK Cells." ImmunoHorizons, vol. 1, no. 2, 2017, pp. 2-9. |
| Miah, S. M. Shahjahan, Jayasuriya, Chathuraka T., Salter, Alexander I., Reilly, Emma C., Fugere, Céline, Yang, Wentian, Chen, Qian, Brossay, Laurent. "Ptpn11 Deletion in CD4+ Cells Does Not Affect T Cell Development and Functions but Causes Cartilage Tumors in a T Cell-Independent Manner." Frontiers in Immunology, vol. 8, 2017, pp. 1326. |
| Erick TK, Anderson CK, Reilly EC, Wands JR, Brossay L. "NFIL3 Expression Distinguishes Tissue-Resident NK Cells and Conventional NK-like Cells in the Mouse Submandibular Glands." The Journal of Immunology, vol. 197, no. 6, 2016, pp. 2485-91. |
| Erick, Timothy K, Brossay, Laurent. "Phenotype and functions of conventional and non-conventional NK cells." Current Opinion in Immunology, vol. 38, 2016, pp. 67-74. |
| Anderson CK, Brossay L. "The role of MHC class Ib-restricted T cells during infection." Immunogenetics, vol. 68, no. 8, 2016, pp. 677-91. |
| Anderson, C. K., Salter, A. I., Toussaint, L. E., Reilly, E. C., Fugere, C., Srivastava, N., Kerr, W. G., Brossay, L. "Role of SHIP1 in Invariant NKT Cell Development and Functions." The Journal of Immunology, vol. 195, no. 5, 2015, pp. 2149-2156. |
| Guan, Jean, Miah, S. M. Shahjahan, Wilson, Zachary S., Erick, Timothy K., Banh, Cindy, Brossay, Laurent. "Role of Type I Interferon Receptor Signaling on NK Cell Development and Functions." PLoS ONE, vol. 9, no. 10, 2014, pp. e111302. |
| Reilly EC, Thompson EA, Aspeslagh S, Wands JR, Elewaut D, Brossay L. "Activated iNKT cells promote memory CD8+ T cell differentiation during viral infection." PLoS ONE, vol. 7, no. 5, 2012, pp. e37991. |
| Case LK, Toussaint L, Moussawi M, Roberts B, Saligrama N, Brossay L, Huber SA, Teuscher C. "Chromosome y regulates survival following murine coxsackievirus b3 infection." G3 (Bethesda, Md.), vol. 2, no. 1, 2012, pp. 115-21. |
| Banh C, Miah SM, Kerr WG, Brossay L. "Mouse natural killer cell development and maturation are differentially regulated by SHIP-1." Blood, vol. 120, no. 23, 2012, pp. 4583-90. |
| Vivier E, Ugolini S, Blaise D, Chabannon C, Brossay L. "Targeting natural killer cells and natural killer T cells in cancer." Nature reviews. Immunology, vol. 12, no. 4, 2012, pp. 239-52. |
| Schlub TE, Sun JC, Walton SM, Robbins SH, Pinto AK, Munks MW, Hill AB, Brossay L, Oxenius A, Davenport MP. "Comparing the kinetics of NK cells, CD4, and CD8 T cells in murine cytomegalovirus infection." The Journal of Immunology, vol. 187, no. 3, 2011, pp. 1385-92. |
| Tessmer MS, Reilly EC, Brossay L. "Salivary gland NK cells are phenotypically and functionally unique." PLoS pathogens, vol. 7, no. 1, 2011, pp. e1001254. |
| Terrizzi SC, Banh C, Brossay L. "A protocol for the production of KLRG1 tetramer." Journal of visualized experiments : JoVE, no. 35, 2010. |
| Reilly EC, Wands JR, Brossay L. "Cytokine dependent and independent iNKT cell activation." Cytokine, vol. 51, no. 3, 2010, pp. 227-31. |
| Nguyen V, Cao L, Lin JT, Hung N, Ritz A, Yu K, Jianu R, Ulin SP, Raphael BJ, Laidlaw DH, Brossay L, Salomon AR. "A new approach for quantitative phosphoproteomic dissection of signaling pathways applied to T cell receptor activation." Mol Cell Proteomics, vol. 8, no. 11, 2009, pp. 2418-31. |
| Banh, Cindy, Brossay, Laurent. "Immune Receptors, Cadherins and their Interactions." CIR, vol. 5, no. 1, 2009, pp. 2-9. |
| Banh C, Fugère C, Brossay L. "Immunoregulatory functions of KLRG1 cadherin interactions are dependent on forward and reverse signaling." Blood, vol. 114, no. 26, 2009, pp. 5299-306. |
| Wintermeyer P, Cheng CW, Gehring S, Hoffman BL, Holub M, Brossay L, Gregory SH. "Invariant natural killer T cells suppress the neutrophil inflammatory response in a mouse model of cholestatic liver damage." Gastroenterology, vol. 136, no. 3, 2009, pp. 1048-59. |
| Tessmer MS, Fatima A, Paget C, Trottein F, Brossay L. "NKT cell immune responses to viral infection." Expert Opinion on Therapeutic Targets, vol. 13, no. 2, 2009, pp. 153-62. |
| Guia S, Cognet C, de Beaucoudrey L, Tessmer MS, Jouanguy E, Berger C, Filipe-Santos O, Feinberg J, Camcioglu Y, Levy J, Al Jumaah S, Al-Hajjar S, Stephan JL, Fieschi C, Abel L, Brossay L, Casanova JL, Vivier E. "A role for interleukin-12/23 in the maturation of human natural killer and CD56+ T cells in vivo." Blood, vol. 111, no. 10, 2008, pp. 5008-16. |
| Chaix J, Tessmer MS, Hoebe K, Fuséri N, Ryffel B, Dalod M, Alexopoulou L, Beutler B, Brossay L, Vivier E, Walzer T. "Cutting edge: Priming of NK cells by IL-18." The Journal of Immunology, vol. 181, no. 3, 2008, pp. 1627-31. |
| Wesley JD, Tessmer MS, Chaukos D, Brossay L. "NK cell-like behavior of Valpha14i NK T cells during MCMV infection." PLoS pathogens, vol. 4, no. 7, 2008, pp. e1000106. |
| Pilbeam K, Basse P, Brossay L, Vujanovic N, Gerstein R, Vallejo AN, Borghesi L. "The ontogeny and fate of NK cells marked by permanent DNA rearrangements." The Journal of Immunology, vol. 180, no. 3, 2008, pp. 1432-41. |
| Wesley JD, Tessmer MS, Paget C, Trottein F, Brossay L. "A Y chromosome-linked factor impairs NK T development." The Journal of Immunology, vol. 179, no. 6, 2007, pp. 3480-7. |
| Tessmer MS, Fugere C, Stevenaert F, Naidenko OV, Chong HJ, Leclercq G, Brossay L. "KLRG1 binds cadherins and preferentially associates with SHIP-1." International immunology, vol. 19, no. 4, 2007, pp. 391-400. |
| Wesley JD, Robbins SH, Sidobre S, Kronenberg M, Terrizzi S, Brossay L. "Cutting edge: IFN-gamma signaling to macrophages is required for optimal Valpha14i NK T/NK cell cross-talk." The Journal of Immunology, vol. 174, no. 7, 2005, pp. 3864-8. |
| Robbins SH, Tessmer MS, Van Kaer L, Brossay L. "Direct effects of T-bet and MHC class I expression, but not STAT1, on peripheral NK cell maturation." European Journal of Immunology, vol. 35, no. 3, 2005, pp. 757-65. |
| Lawton AP, Prigozy TI, Brossay L, Pei B, Khurana A, Martin D, Zhu T, Späte K, Ozga M, Höning S, Bakke O, Kronenberg M. "The mouse CD1d cytoplasmic tail mediates CD1d trafficking and antigen presentation by adaptor protein 3-dependent and -independent mechanisms." The Journal of Immunology, vol. 174, no. 6, 2005, pp. 3179-86. |
| Eash S, Tavares R, Stopa EG, Robbins SH, Brossay L, Atwood WJ. "Differential distribution of the JC virus receptor-type sialic acid in normal human tissues." The American Journal of Pathology, vol. 164, no. 2, 2004, pp. 419-28. |
| Robbins SH, Tessmer MS, Mikayama T, Brossay L. "Expansion and contraction of the NK cell compartment in response to murine cytomegalovirus infection." The Journal of Immunology, vol. 173, no. 1, 2004, pp. 259-66. |
| Anfossi N, Robbins SH, Ugolini S, Georgel P, Hoebe K, Bouneaud C, Ronet C, Kaser A, DiCioccio CB, Tomasello E, Blumberg RS, Beutler B, Reiner SL, Alexopoulou L, Lantz O, Raulet DH, Brossay L, Vivier E. "Expansion and function of CD8+ T cells expressing Ly49 inhibitory receptors specific for MHC class I molecules." The Journal of Immunology, vol. 173, no. 6, 2004, pp. 3773-82. |
| Maverakis E, Beech J, Stevens DB, Ametani A, Brossay L, van den Elzen P, Mendoza R, Thai Q, Macias LH, Ethell D, Campagnoni CW, Campagnoni AT, Sette A, Sercarz EE. "Autoreactive T cells can be protected from tolerance induction through competition by flanking determinants for access to class II MHC." Proceedings of the National Academy of Sciences, vol. 100, no. 9, 2003, pp. 5342-7. |
| Robbins SH, Terrizzi SC, Sydora BC, Mikayama T, Brossay L. "Differential regulation of killer cell lectin-like receptor G1 expression on T cells." The Journal of Immunology, vol. 170, no. 12, 2003, pp. 5876-85. |
| Rhee RJ, Carlton S, Lomas JL, Lane C, Brossay L, Cioffi WG, Ayala A. "Inhibition of CD1d activation suppresses septic mortality: a role for NK-T cells in septic immune dysfunction." Journal of Surgical Research, vol. 115, no. 1, 2003, pp. 74-81. |
| Wesley J, Brossay L. "NK T cell-NK cell cross-talk: reciprocal interaction and activation?." Archivum immunologiae et therapiae experimentalis, vol. 51, no. 2, 2003, pp. 121-6. |
| Robbins SH, Nguyen KB, Takahashi N, Mikayama T, Biron CA, Brossay L. "Cutting edge: inhibitory functions of the killer cell lectin-like receptor G1 molecule during the activation of mouse NK cells." The Journal of Immunology, vol. 168, no. 6, 2002, pp. 2585-9. |
| Robbins SH, Brossay L. "NK cell receptors: emerging roles in host defense against infectious agents." Microbes and infection / Institut Pasteur, vol. 4, no. 15, 2002, pp. 1523-30. |
| Kamada N, Iijima H, Kimura K, Harada M, Shimizu E, Motohashi Si, Kawano T, Shinkai H, Nakayama T, Sakai T, Brossay L, Kronenberg M, Taniguchi M. "Crucial amino acid residues of mouse CD1d for glycolipid ligand presentation to V(alpha)14 NKT cells." International immunology, vol. 13, no. 7, 2001, pp. 853-61. |
| Prigozy TI, Naidenko O, Qasba P, Elewaut D, Brossay L, Khurana A, Natori T, Koezuka Y, Kulkarni A, Kronenberg M. "Glycolipid antigen processing for presentation by CD1d molecules." Science, vol. 291, no. 5504, 2001, pp. 664-7. |
| Biron CA, Brossay L. "NK cells and NKT cells in innate defense against viral infections." Current Opinion in Immunology, vol. 13, no. 4, 2001, pp. 458-64. |
| Elewaut D, Brossay L, Santee SM, Naidenko OV, Burdin N, De Winter H, Matsuda J, Ware CF, Cheroutre H, Kronenberg M. "Membrane lymphotoxin is required for the development of different subpopulations of NK T cells." The Journal of Immunology, vol. 165, no. 2, 2000, pp. 671-9. |
| Burdin N, Brossay L, Degano M, Iijima H, Gui M, Wilson IA, Kronenberg M. "Structural requirements for antigen presentation by mouse CD1." Proceedings of the National Academy of Sciences, vol. 97, no. 18, 2000, pp. 10156-61. |
| Kronenberg M, Brossay L, Kurepa Z, Forman J. "Conserved lipid and peptide presentation functions of nonclassical class I molecules." Immunology today, vol. 20, no. 11, 1999, pp. 515-21. |
| Szalay G, Ladel CH, Blum C, Brossay L, Kronenberg M, Kaufmann SH. "Cutting edge: anti-CD1 monoclonal antibody treatment reverses the production patterns of TGF-beta 2 and Th1 cytokines and ameliorates listeriosis in mice." The Journal of Immunology, vol. 162, no. 12, 1999, pp. 6955-8. |
| Brossay L, Kronenberg M. "Highly conserved antigen-presenting function of CD1d molecules." Immunogenetics, vol. 50, no. 3-4, 1999, pp. 146-51. |
| Burdin N, Brossay L, Kronenberg M. "Immunization with alpha-galactosylceramide polarizes CD1-reactive NK T cells towards Th2 cytokine synthesis." European Journal of Immunology, vol. 29, no. 6, 1999, pp. 2014-25. |
| Chang CS, Brossay L, Kronenberg M, Kane KP. "The murine nonclassical class I major histocompatibility complex-like CD1.1 molecule protects target cells from lymphokine-activated killer cell cytolysis." Journal of Experimental Medicine, vol. 189, no. 3, 1999, pp. 483-91. |
| Brossay L, Burdin N, Tangri S, Kronenberg M. "Antigen-presenting function of mouse CD1: one molecule with two different kinds of antigenic ligands." Immunological reviews, vol. 163, 1998, pp. 139-50. |
| Amano M, Baumgarth N, Dick MD, Brossay L, Kronenberg M, Herzenberg LA, Strober S. "CD1 expression defines subsets of follicular and marginal zone B cells in the spleen: beta 2-microglobulin-dependent and independent forms." The Journal of Immunology, vol. 161, no. 4, 1998, pp. 1710-7. |
| Brossay L, Chioda M, Burdin N, Koezuka Y, Casorati G, Dellabona P, Kronenberg M. "CD1d-mediated recognition of an alpha-galactosylceramide by natural killer T cells is highly conserved through mammalian evolution." Journal of Experimental Medicine, vol. 188, no. 8, 1998, pp. 1521-8. |
| Couedel C, Peyrat MA, Brossay L, Koezuka Y, Porcelli SA, Davodeau F, Bonneville M. "Diverse CD1d-restricted reactivity patterns of human T cells bearing "invariant" AV24BV11 TCR." European Journal of Immunology, vol. 28, no. 12, 1998, pp. 4391-7. |
| Brossay L, Tangri S, Bix M, Cardell S, Locksley R, Kronenberg M. "Mouse CD1-autoreactive T cells have diverse patterns of reactivity to CD1+ targets." The Journal of Immunology, vol. 160, no. 8, 1998, pp. 3681-8. |
| Wolfgang M, Lauer P, Park HS, Brossay L, Hébert J, Koomey M. "PilT mutations lead to simultaneous defects in competence for natural transformation and twitching motility in piliated Neisseria gonorrhoeae." Molecular Microbiology, vol. 29, no. 1, 1998, pp. 321-30. |
| Tangri S, Brossay L, Burdin N, Lee DJ, Corr M, Kronenberg M. "Presentation of peptide antigens by mouse CD1 requires endosomal localization and protein antigen processing." Proceedings of the National Academy of Sciences, vol. 95, no. 24, 1998, pp. 14314-9. |
| Burdin N, Brossay L, Koezuka Y, Smiley ST, Grusby MJ, Gui M, Taniguchi M, Hayakawa K, Kronenberg M. "Selective ability of mouse CD1 to present glycolipids: alpha-galactosylceramide specifically stimulates V alpha 14+ NK T lymphocytes." The Journal of Immunology, vol. 161, no. 7, 1998, pp. 3271-81. |
| Brossay L, Naidenko O, Burdin N, Matsuda J, Sakai T, Kronenberg M. "Structural requirements for galactosylceramide recognition by CD1-restricted NK T cells." The Journal of Immunology, vol. 161, no. 10, 1998, pp. 5124-8. |
| Brossay L, Jullien D, Cardell S, Sydora BC, Burdin N, Modlin RL, Kronenberg M. "Mouse CD1 is mainly expressed on hemopoietic-derived cells." The Journal of Immunology, vol. 159, no. 3, 1997, pp. 1216-24. |
| Teitell M, Holcombe HR, Brossay L, Hagenbaugh A, Jackson MJ, Pond L, Balk SP, Terhorst C, Peterson PA, Kronenberg M. "Nonclassical behavior of the mouse CD1 class I-like molecule." The Journal of Immunology, vol. 158, no. 5, 1997, pp. 2143-9. |
| Jullien D, Brossay L, Sieling PA, Modlin RL, Kronenberg M. "CD1: clues on a new antigen-presenting pathway." Research in immunology, vol. 147, no. 5, 1996, pp. 321-8. |
| Sydora BC, Brossay L, Hagenbaugh A, Kronenberg M, Cheroutre H. "TAP-independent selection of CD8+ intestinal intraepithelial lymphocytes." The Journal of Immunology, vol. 156, no. 11, 1996, pp. 4209-16. |
| Villeneuve A, Brossay L, Paradis G, Hébert J. "Characterization of the humoral response induced by a synthetic peptide of the major outer membrane protein of Chlamydia trachomatis serovar B." Infection and Immunity, vol. 62, no. 8, 1994, pp. 3547-9. |
| Villeneuve A, Brossay L, Paradis G, Hébert J. "Determination of neutralizing epitopes in variable domains I and IV of the major outer-membrane protein from Chlamydia trachomatis serovar K." Microbiology (Reading, England), vol. 140 ( Pt 9), 1994, pp. 2481-7. |
| Brossay L, Paradis G, Fox R, Koomey M, Hébert J. "Identification, localization, and distribution of the PilT protein in Neisseria gonorrhoeae." Infection and Immunity, vol. 62, no. 6, 1994, pp. 2302-8. |
| Brossay L, Villeneuve A, Paradis G, Coté L, Mourad W, Hébert J. "Mimicry of a neutralizing epitope of the major outer membrane protein of Chlamydia trachomatis by anti-idiotypic antibodies." Infection and Immunity, vol. 62, no. 2, 1994, pp. 341-7. |
| Brossay L, Paradis G, Pépin A, Mourad W, Coté L, Hébert J. "Idiotype and anti-anti-idiotype antibodies to Neisseria gonorrhoeae lipooligosaccharides with bactericidal activity but no cross-reactivity with red blood cell antigens." The Journal of Immunology, vol. 151, no. 1, 1993, pp. 234-43. |
Development and functions of tissue resident NK cells
Cytomegalovirus (CMV) infects between 60 to 70% of adults. In immunocompetent individuals, infection often goes unnoticed and is cleared from most organs. However, CMV persists and eventually goes into a latent phase in the salivary glands. Reactivation of CMV in immunocompromised individuals and neonate infections can be life threatening. We are currently investigating why Natural Killer (NK) cells are poor immune effectors in the salivary glands, presumably leading to CMV persistence in this organ.
Role of phosphatases during NK cell and iNKT cell development
NK cell and NKT cell inhibitory receptors associate predominantly with the tyrosine phosphatase SHP-1 or SHP-2. Using mice conditionally deficient for these two phosphatases, we are currently examining their roles during NK and iNKT cell development. We are also investigating how these phosphatases regulate NK cell and iNKT cell immune responses.
Non-classical T cell response to viral infection
In the last 30 years significant progress has been made regarding the role and functions of CD4+ and CD8+conventional T cells. Much less is known about non-classical T cells, which include iNKT cells, MAIT cells as well as M3 and Qa-1 restricted T cells. We take advantage of a variety of tools including MCMV mutants and immunodeficient mice to determine the role of these understudied cells.
- NIH Grant RO1 AI46709 (15-19); NK T cell interaction with NK cells during viral infections. 05/08/2018 - 04/30/2025
- NIH Grant RO1 AI173163 (1-5); Immune response to MCMV infection in the salivary glands, 05/12/2023 - 06/30/2028
| Year | Degree | Institution |
|---|---|---|
| 1994 | PhD | Universite Laval |
| 1988 | MS | Université de Rennes 1 |
| 1986 | BS | Université de Rennes 1 |
Professional Memberships
American Association of Immunologists
The Society for Natural Immunity
| BIOL 0530 - Principles of Immunology |
| BIOL 1520 - Innate Immunity |
