Her research focus is on understanding the aggressiveness of triple-negative breast cancers, and to identify targets and agents to overcome resistance mechanisms. During her graduate training she worked on the identification of protein complexes that were important in breast cancer growth and estrogen hypersensitivity. For her postdoctoral training Dr. den Hollander collaborated with the Bioinformatics Research Lab, and the Genome Sequencing Center at Baylor College of Medicine, in the development of a new high-throughput sequencing technique to aid in the discovery of genetic alterations in cancer. In the Department of Clinical Cancer Prevention at MD Anderson, Dr. den Hollander used preclinical animal models for the development of new strategies in triple-negative breast cancer prevention and treatment. Her current research is focused on identifying the underlying mechanisms of the induction and maintenance of cancer stem cells and their resistance mechanisms using single cell ‘omics’ techniques identifying targets to successfully treat cancers and prevent metastatic progression.
| Deshmukh AP, den Hollander P, Kuburich NA, Vasaikar S, Joseph R, Mani SA. "Enrichment of Cancer Stem Cells in a Tumorsphere Assay." Methods in Molecular Biology, vol. 2429, 2022, pp. 501-507. |
| Castaneda, Maria, Hollander, Petra den, Mani, Sendurai A. "Forkhead Box Transcription Factors: Double-Edged Swords in Cancer." Cancer Research, vol. 82, no. 11, 2022, pp. 2057-2065. |
| Kuburich NA, den Hollander P, Deshmukh AP, Vasaikar S, Joseph R, Wicha MS, Mani SA. "In Vitro Quantification of Cancer Stem Cells Using a Mammosphere Formation Assay." Methods in Molecular Biology, vol. 2429, 2022, pp. 509-513. |
| den Hollander P, Joseph R, Vasaikar S, Kuburich NA, Deshmukh AP, Mani SA. "Limiting Dilution Tumor Initiation Assay: An In Vivo Approach for the Study of Cancer Stem Cells." Methods in Molecular Biology, vol. 2429, 2022, pp. 547-554. |
| Castaneda M, den Hollander P, Kuburich NA, Rosen JM, Mani SA. "Mechanisms of cancer metastasis." Seminars in Cancer Biology, vol. 87, 2022, pp. 17-31. |
| Kudaravalli S, den Hollander P, Mani SA. "Role of p38 MAP kinase in cancer stem cells and metastasis." Oncogene, vol. 41, no. 23, 2022, pp. 3177-3185. |
| Kuburich, Nick A., den Hollander, Petra, Pietz, Jordan T., Mani, Sendurai A. "Vimentin and cytokeratin: Good alone, bad together." Seminars in Cancer Biology, vol. 86, no. Pt 3, 2022, pp. 816-826. |
| den Hollander, Petra, Vasaikar, Suhas, Castaneda, Maria, Joseph, Robiya, Deshmukh, Abhijeet P., Zhou, Tieling, Pietila, Mika, Fu, Chunxiao, Symmans, William F., Soundararajan, Rama, Mani, Sendurai A. "Acquisition of cancer stem cell properties during EMT requires cell division." [], 2021. |
| Bushnell GG, Deshmukh AP, den Hollander P, Luo M, Soundararajan R, Jia D, Levine H, Mani SA, Wicha MS. "Breast cancer dormancy: need for clinically relevant models to address current gaps in knowledge." npj Breast Cancer, vol. 7, no. 1, 2021, pp. 66. |
| Joseph, Robiya, Soundararajan, Rama, Vasaikar, Suhas, Yang, Fei, Allton, Kendra L., Tian, Lin, den Hollander, Petra, Isgandarova, Sevinj, Haemmerle, Monika, Mino, Barbara, Zhou, Tieling, Shin, Crystal, Martinez-Paniagua, Melisa, Sahin, Aysegul A., Rodriguez-Canales, Jaime, Gelovani, Juri, Chang, Jeffrey T., Acharya, Ghanashyam, Sood, Anil K., Wistuba, Ignacio I., Gibbons, Don L., Solis, Luisa M., Barton, Michelle C., Varadarajan, Navin, Rosen, Jeffrey M., Zhang, Xiang H., Mani, Sendurai A. "CD8+ T cells inhibit metastasis and CXCL4 regulates its function." British Journal of Cancer, vol. 125, no. 2, 2021, pp. 176-189. |
| Deshmukh, Abhijeet P., Vasaikar, Suhas V., Tomczak, Katarzyna, Tripathi, Shubham, den Hollander, Petra, Arslan, Emre, Chakraborty, Priyanka, Soundararajan, Rama, Jolly, Mohit Kumar, Rai, Kunal, Levine, Herbert, Mani, Sendurai A. "Identification of EMT signaling cross-talk and gene regulatory networks by single-cell RNA sequencing." Proceedings of the National Academy of Sciences, vol. 118, no. 19, 2021. |
| Vasaikar SV, Deshmukh AP, den Hollander P, Addanki S, Kuburich NA, Kudaravalli S, Joseph R, Chang JT, Soundararajan R, Mani SA. "EMTome: a resource for pan-cancer analysis of epithelial-mesenchymal transition genes and signatures." British Journal of Cancer, vol. 124, no. 1, 2020, pp. 259-269. |
| Vijay, Geraldine Vidhya, Zhao, Na, Den Hollander, Petra, Toneff, Mike J., Joseph, Robiya, Pietila, Mika, Taube, Joseph H., Sarkar, Tapasree R., Ramirez-Pena, Esmeralda, Werden, Steven J., Shariati, Maryam, Gao, Ruli, Sobieski, Mary, Stephan, Clifford C., Sphyris, Nathalie, Miura, Noayuki, Davies, Peter, Chang, Jeffrey T., Soundararajan, Rama, Rosen, Jeffrey M., Mani, Sendurai A. "GSK3β regulates epithelial-mesenchymal transition and cancer stem cell properties in triple-negative breast cancer." Breast Cancer Research, vol. 21, no. 1, 2019, pp. 37. |
| Ramirez-Peña, Esmeralda, Arnold, James, Shivakumar, Vinita, Joseph, Robiya, Vidhya Vijay, Geraldine, den Hollander, Petra, Bhangre, Neeraja, Allegakoen, Paul, Prasad, Rishika, Conley, Zachary, Matés, José M., Márquez, Javier, Chang, Jeffrey T., Vasaikar, Suhas, Soundararajan, Rama, Sreekumar, Arun, Mani, Sendurai A. "The Epithelial to Mesenchymal Transition Promotes Glutamine Independence by Suppressing GLS2 Expression." Cancers, vol. 11, no. 10, 2019, pp. 1610. |
| Jia D, Jolly MK, Tripathi SC, Den Hollander P, Huang B, Lu M, Celiktas M, Ramirez-Peña E, Ben-Jacob E, Onuchic JN, Hanash SM, Mani SA, Levine H. "Distinguishing mechanisms underlying EMT tristability." Cancer Convergence, vol. 1, no. 1, 2017, pp. 2. |
| Mazumdar, Abhijit, Poage, Graham M., Shepherd, Jonathan, Tsimelzon, Anna, Hartman, Zachary C., Den Hollander, Petra, Hill, Jamal, Zhang, Yun, Chang, Jenny, Hilsenbeck, Susan G., Fuqua, Suzanne, Kent Osborne, C., Mills, Gordon B., Brown, Powel H. "Analysis of phosphatases in ER-negative breast cancers identifies DUSP4 as a critical regulator of growth and invasion." Breast Cancer Research and Treatment, vol. 158, no. 3, 2016, pp. 441-454. |
| den Hollander, Petra, Rawls, Kathryn, Tsimelzon, Anna, Shepherd, Jonathan, Mazumdar, Abhijit, Hill, Jamal, Fuqua, Suzanne A.W., Chang, Jenny C., Osborne, C. Kent, Hilsenbeck, Susan G., Mills, Gordon B., Brown, Powel H. "Phosphatase PTP4A3 Promotes Triple-Negative Breast Cancer Growth and Predicts Poor Patient Survival." Cancer Research, vol. 76, no. 7, 2016, pp. 1942-1953. |
| Werden, S J, Sphyris, N, Sarkar, T R, Paranjape, A N, LaBaff, A M, Taube, J H, Hollier, B G, Ramirez-Peña, E Q, Soundararajan, R, den Hollander, P, Powell, E, Echeverria, G V, Miura, N, Chang, J T, Piwnica-Worms, H, Rosen, J M, Mani, S A. "Phosphorylation of serine 367 of FOXC2 by p38 regulates ZEB1 and breast cancer metastasis, without impacting primary tumor growth." Oncogene, vol. 35, no. 46, 2016, pp. 5977-5988. |
| Toneff, M. J., Sreekumar, A., Tinnirello, A., Hollander, P. Den, Habib, S., Li, S., Ellis, M. J., Xin, L., Mani, S. A., Rosen, J. M. "The Z-cad dual fluorescent sensor detects dynamic changes between the epithelial and mesenchymal cellular states." BMC Biology, vol. 14, no. 1, 2016, pp. 47. |
| Hollander, Petra den, Brown, Powel H. "Advances in Targeted Therapy for the Prevention of Breast Cancer." Breast Diseases: A Year Book Quarterly, vol. 24, no. 4, 2013, pp. 309-314. |
| Hartman, Zachary C., Poage, Graham M., den Hollander, Petra, Tsimelzon, Anna, Hill, Jamal, Panupinthu, Nattapon, Zhang, Yun, Mazumdar, Abhijit, Hilsenbeck, Susan G., Mills, Gordon B., Brown, Powel H. "Growth of Triple-Negative Breast Cancer Cells Relies upon Coordinate Autocrine Expression of the Proinflammatory Cytokines IL-6 and IL-8." Cancer Research, vol. 73, no. 11, 2013, pp. 3470-3480. |
| den Hollander, Petra, Savage, Michelle I., Brown, Powel H. "Targeted Therapy for Breast Cancer Prevention." Frontiers in Oncology, vol. 3, 2013. |
| Hampton, Oliver A., Miller, Christopher A., Koriabine, Maxim, Li, Jian, Den Hollander, Petra, Carbone, Lucia, Nefedov, Mikhail, Ten Hallers, Boudewijn F.H., Lee, Adrian V., De Jong, Pieter J., Milosavljevic, Aleksandar. "Long-range massively parallel mate pair sequencing detects distinct mutations and similar patterns of structural mutability in two breast cancer cell lines." Cancer Genetics, vol. 204, no. 8, 2011, pp. 447-457. |
| Hampton, Oliver A., Den Hollander, Petra, Miller, Christopher A., Delgado, David A., Li, Jian, Coarfa, Cristian, Harris, Ronald A., Richards, Stephen, Scherer, Steven E., Muzny, Donna M., Gibbs, Richard A., Lee, Adrian V., Milosavljevic, Aleksandar. "A sequence-level map of chromosomal breakpoints in the MCF-7 breast cancer cell line yields insights into the evolution of a cancer genome." Genome Research, vol. 19, no. 2, 2008, pp. 167-177. |
| den Hollander, Petra, Rayala, Suresh K., Coverley, Dawn, Kumar, Rakesh. "Ciz1, a Novel DNA-Binding Coactivator of the Estrogen Receptor α, Confers Hypersensitivity to Estrogen Action." Cancer Research, vol. 66, no. 22, 2006, pp. 11021-11029. |
| den Hollander, Petra, Kumar, Rakesh. "Dynein Light Chain 1 Contributes to Cell Cycle Progression by Increasing Cyclin-Dependent Kinase 2 Activity in Estrogen-Stimulated Cells." Cancer Research, vol. 66, no. 11, 2006, pp. 5941-5949. |
| Rayala, Suresh K, den Hollander, Petra, Balasenthil, Seetharaman, Yang, Zhibo, Broaddus, Russell R, Kumar, Rakesh. "Functional regulation of oestrogen receptor pathway by the dynein light chain 1." EMBO reports, vol. 6, no. 6, 2005, pp. 538-544. |
| Vadlamudi, Ratna K, Bagheri-Yarmand, Rozita, Yang, Zhibo, Balasenthil, Seetharaman, Nguyen, Diep, Sahin, Aysegul A, den Hollander, Petra, Kumar, Rakesh. "Dynein light chain 1, a p21-activated kinase 1-interacting substrate, promotes cancerous phenotypes." Cancer Cell, vol. 5, no. 6, 2004, pp. 575-585. |
R01 CA155243
Pleiotropic functions of FOXC2 in EMT stem cells and breast cancer progression
Project Goal: To understand the role of the transcription factor FOXC2 in mediating EMT and stem cell properties in cancer and normal mammary gland development.
Role: Co-investigator
R01 CA200970
Preclinical characterization of EMT/CSC-specific small molecule inhibitors for TNBCs
Project Goal: To discover and develop novel small molecule inhibitors for specific activity against TNBCs.
Role: Co-investigator
R01 CA262106 (PI:SA Mani);
Vimentin Phospho-Malleability is Critical for Maintaining Stemness and Metastatic Properties
Project Goal: To examine the significance of vimentin phospho-malleability on EMT and stem cell properties in the context of breast tumor metastasis.
Role: Co-investigator
| Year | Degree | Institution |
|---|---|---|
| 2006 | PhD | University of Texas |
| 2002 | MSc | Vrije Universiteit Amsterdam |
| Postdoctoral Fellow | MD Anderson Hospital & Tumor Institute of the University of Texas Cancer Center | 2010-2013 | |
| Postdoctoral Associate | Baylor College of Medicine | 2007-2010 |
| Assistant Professor. MD Anderson Hospital & Tumor Institute of the University of Texas Cancer Center, 2022-2022 |
| Instructor. MD Anderson Hospital & Tumor Institute of the University of Texas Cancer Center, 2013-2022 |
