Dr. Reichner is a Professor in the Department of Surgery at the Brown University Medical School, Providence, RI. He received his BS from the Ohio State University and his Ph.D. in Microbiology and Immunology from the State University of New York at Buffalo in 1983. He completed postdoctoral work at MD Anderson Hospital & Tumor Institute of the University of Texas Cancer Center and the Johns Hopkins School of Medicine.
| Wilson ZS, Witt H, Hazlett L, Harman M, Neumann BM, Whitman A, Patel M, Ross RS, Franck C, Reichner JS, Lefort CT. "Context-Dependent Role of Vinculin in Neutrophil Adhesion, Motility and Trafficking." Scientific reports, vol. 10, no. 1, 2020, pp. 2142. |
| Hazlett L, Landauer AK, Patel M, Witt HA, Yang J, Reichner JS, Franck C. "Epifluorescence-based three-dimensional traction force microscopy." Scientific reports, vol. 10, no. 1, 2020, pp. 16599. |
| Byrd AS, Carmona-Rivera C, O'Neil LJ, Carlucci PM, Cisar C, Rosenberg AZ, Kerns ML, Caffrey JA, Milner SM, Sacks JM, Aliu O, Broderick KP, Reichner JS, Miller LS, Kang S, Robinson WH, Okoye GA, Kaplan MJ. "Neutrophil extracellular traps, B cells, and type I interferons contribute to immune dysregulation in hidradenitis suppurativa." Science Translational Medicine, vol. 11, no. 508, 2019. |
| Dickinson, Catherine M., LeBlanc, Brian W., Edhi, Muhammad M., Heffernan, Daithi S., Faridi, Mohd. Hafeez, Gupta, Vineet, Cioffi, William G., O’Brien, Xian, Reichner, Jonathan S. "Leukadherin-1 ameliorates endothelial barrier damage mediated by neutrophils from critically ill patients." Journal of Intensive Care, vol. 6, no. 1, 2018, pp. 19. |
| Biron, Bethany M., Chung, Chun-Shiang, Chen, Yaping, Wilson, Zachary, Fallon, Eleanor A., Reichner, Jonathan S., Ayala, Alfred. "PAD4 Deficiency Leads to Decreased Organ Dysfunction and Improved Survival in a Dual Insult Model of Hemorrhagic Shock and Sepsis." The Journal of Immunology, vol. 200, no. 5, 2018, pp. ji1700639. |
| Faridi MH, Khan SQ, Zhao W, Lee HW, Altintas MM, Zhang K, Kumar V, Armstrong AR, Carmona-Rivera C, Dorschner JM, Schnaith AM, Li X, Ghodke-Puranik Y, Moore E, Purmalek M, Irizarry-Caro J, Zhang T, Day R, Stoub D, Hoffmann V, Khaliqdina SJ, Bhargava P, Santander AM, Torroella-Kouri M, Issac B, Cimbaluk DJ, Zloza A, Prabhakar R, Deep S, Jolly M, Koh KH, Reichner JS, Bradshaw EM, Chen J, Moita LF, Yuen PS, Li Tsai W, Singh B, Reiser J, Nath SK, Niewold TB, Vazquez-Padron RI, Kaplan MJ, Gupta V. "CD11b activation suppresses TLR-dependent inflammation and autoimmunity in systemic lupus erythematosus." Journal of Clinical Investigation, vol. 127, no. 4, 2017, pp. 1271-1283. |
| Biron BM, Chung CS, O'Brien XM, Chen Y, Reichner JS, Ayala A. "Cl-Amidine Prevents Histone 3 Citrullination and Neutrophil Extracellular Trap Formation, and Improves Survival in a Murine Sepsis Model." Journal of innate immunity, vol. 9, no. 1, 2017, pp. 22-32. |
| O'Brien XM, Biron BM, Reichner JS. "Consequences of extracellular trap formation in sepsis." Current opinion in hematology, vol. 24, no. 1, 2017, pp. 66-71. |
| Johnson CM, O'Brien XM, Byrd AS, Parisi VE, Loosely AJ, Li W, Witt H, Faridi MH, LeFort CT, Gupta V, Kim M, Reichner JS. "Integrin Cross-Talk Regulates the Human Neutrophil Response to Fungal β-Glucan in the Context of the Extracellular Matrix: A Prominent Role for VLA3 in the Antifungal Response." The Journal of Immunology, vol. 198, no. 1, 2017, pp. 318-334. |
| Stout, David A., Bar-Kochba, Eyal, Estrada, Jonathan B., Toyjanova, Jennet, Kesari, Haneesh, Reichner, Jonathan S., Franck, Christian. "Mean deformation metrics for quantifying 3D cell–matrix interactions without requiring information about matrix material properties." Proceedings of the National Academy of Sciences, vol. 113, no. 11, 2016, pp. 2898-2903. |
| O'Brien XM, Reichner JS. "Neutrophil Integrins and Matrix Ligands and NET Release." Frontiers in immunology, vol. 7, 2016, pp. 363. |
| Loosley AJ, O'Brien XM, Reichner JS, Tang JX. "Describing directional cell migration with a characteristic directionality time." PLoS ONE, vol. 10, no. 5, 2015, pp. e0127425. |
| Toyjanova J, Flores-Cortez E, Reichner JS, Franck C. "Matrix confinement plays a pivotal role in regulating neutrophil-generated tractions, speed, and integrin utilization." Journal of Biological Chemistry, vol. 290, no. 6, 2015, pp. jbc.M114.619643. |
| Byrd AS, O'Brien XM, Laforce-Nesbitt SS, Parisi VE, Hirakawa MP, Bliss JM, Reichner JS. "NETosis in Neonates: Evidence of a Reactive Oxygen Species-Independent Pathway in Response to Fungal Challenge." The Journal of Infectious Diseases, vol. 213, no. 4, 2015, pp. 634-9. |
| Toyjanova J, Bar-Kochba E, López-Fagundo C, Reichner J, Hoffman-Kim D, Franck C. "High resolution, large deformation 3D traction force microscopy." PLoS ONE, vol. 9, no. 4, 2014, pp. e90976. |
| Ayala A, Elphick GF, Kim YS, Huang X, Carreira-Rosario A, Santos SC, Shubin NJ, Chen Y, Reichner J, Chung CS. "Sepsis-induced potentiation of peritoneal macrophage migration is mitigated by programmed cell death receptor-1 gene deficiency." Journal of innate immunity, vol. 6, no. 3, 2014, pp. 325-38. |
| O'Brien XM, Loosley AJ, Oakley KE, Tang JX, Reichner JS. "Technical advance: introducing a novel metric, directionality time, to quantify human neutrophil chemotaxis as a function of matrix composition and stiffness." Journal of Leukocyte Biology, vol. 95, no. 6, 2014, pp. 993-1004. |
| Magruder HT, Quinn JA, Schwartzbauer JE, Reichner J, Huang A, Filardo EJ. "The G protein-coupled estrogen receptor-1, GPER-1, promotes fibrillogenesis via a Shc-dependent pathway resulting in anchorage-independent growth." Hormones & cancer, vol. 5, no. 6, 2014, pp. 390-404. |
| Byrd AS, O'Brien XM, Johnson CM, Lavigne LM, Reichner JS. "An extracellular matrix-based mechanism of rapid neutrophil extracellular trap formation in response to Candida albicans." The Journal of Immunology, vol. 190, no. 8, 2013, pp. 4136-48. |
| Fox ED, Heffernan DS, Cioffi WG, Reichner JS. "Neutrophils from critically ill septic patients mediate profound loss of endothelial barrier integrity." Critical Care, vol. 17, no. 5, 2013, pp. R226. |
| Brancato SK, Thomay AA, Daley JM, Crane MJ, Reichner JS, Sabo E, Albina JE. "Toll-like receptor 4 signaling regulates the acute local inflammatory response to injury and the fibrosis/neovascularization of sterile wounds." Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, vol. 21, no. 4, 2013, pp. 624-33. |
| O'Brien XM, Heflin KE, Lavigne LM, Yu K, Kim M, Salomon AR, Reichner JS. "Lectin site ligation of CR3 induces conformational changes and signaling." Journal of Biological Chemistry, vol. 287, no. 5, 2012, pp. 3337-48. |
| Newsome CT, Flores E, Ayala A, Gregory S, Reichner JS. "Improved antimicrobial host defense in mice following poly-(1,6)-β-D-glucopyranosyl-(1,3)-β-D-glucopyranose glucan treatment by a gender-dependent immune mechanism." Clinical and vaccine immunology : CVI, vol. 18, no. 12, 2011, pp. 2043-9. |
| Daley JM, Brancato SK, Thomay AA, Reichner JS, Albina JE. "The phenotype of murine wound macrophages." Journal of Leukocyte Biology, vol. 87, no. 1, 2010, pp. 59-67. |
| McMurdy J, Reichner J, Mathews Z, Markey M, Intwala S, Crawford G. "Broadband reflectance spectroscopy for establishing a quantitative metric of vascular leak using the Miles assay." Journal of biomedical optics, vol. 14, no. 5, 2009, pp. 054012. |
| Thomay AA, Daley JM, Sabo E, Worth PJ, Shelton LJ, Harty MW, Reichner JS, Albina JE. "Disruption of interleukin-1 signaling improves the quality of wound healing." The American Journal of Pathology, vol. 174, no. 6, 2009, pp. 2129-36. |
| Muratore CS, Luks FI, Zhou Y, Harty M, Reichner J, Tracy TF. "Endotoxin alters early fetal lung morphogenesis." Journal of Surgical Research, vol. 155, no. 2, 2009, pp. 225-30. |
| Oakes PW, Patel DC, Morin NA, Zitterbart DP, Fabry B, Reichner JS, Tang JX. "Neutrophil morphology and migration are affected by substrate elasticity." Blood, vol. 114, no. 7, 2009, pp. 1387-95. |
| Elphick GF, Sarangi PP, Hyun YM, Hollenbaugh JA, Ayala A, Biffl WL, Chung HL, Rezaie AR, McGrath JL, Topham DJ, Reichner JS, Kim M. "Recombinant human activated protein C inhibits integrin-mediated neutrophil migration." Blood, vol. 113, no. 17, 2009, pp. 4078-85. |
| Morin NA, Oakes PW, Hyun YM, Lee D, Chin YE, King MR, Springer TA, Shimaoka M, Tang JX, Reichner JS, Kim M. "Nonmuscle myosin heavy chain IIA mediates integrin LFA-1 de-adhesion during T lymphocyte migration." Journal of Experimental Medicine, vol. 205, no. 1, 2008, pp. 195-205. |
| Xu XJ, Reichner JS, Mastrofrancesco B, Henry WL Jr, Albina JE. "Prostaglandin E2 suppresses lipopolysaccharide-stimulated IFN-beta production." The Journal of Immunology, vol. 180, no. 4, 2008, pp. 2125-31. |
| Daley JM, Thomay AA, Connolly MD, Reichner JS, Albina JE. "Use of Ly6G-specific monoclonal antibody to deplete neutrophils in mice." Journal of Leukocyte Biology, vol. 83, no. 1, 2008, pp. 64-70. |
| Lavigne LM, O'Brien XM, Kim M, Janowski JW, Albina JE, Reichner JS. "Integrin engagement mediates the human polymorphonuclear leukocyte response to a fungal pathogen-associated molecular pattern." The Journal of Immunology, vol. 178, no. 11, 2007, pp. 7276-82. |
| Lavigne LM, Albina JE, Reichner JS. "Beta-glucan is a fungal determinant for adhesion-dependent human neutrophil functions." The Journal of Immunology, vol. 177, no. 12, 2006, pp. 8667-75. |
| LeBlanc BW, Albina JE, Reichner JS. "The effect of PGG-beta-glucan on neutrophil chemotaxis in vivo." Journal of Leukocyte Biology, vol. 79, no. 4, 2006, pp. 667-75. |
| Albina JE, Mahoney EJ, Daley JM, Wesche DE, Morris SM Jr, Reichner JS. "Macrophage arginase regulation by CCAAT/enhancer-binding protein beta." Shock, vol. 23, no. 2, 2005, pp. 168-72. |
| Daley JM, Reichner JS, Mahoney EJ, Manfield L, Henry WL Jr, Mastrofrancesco B, Albina JE. "Modulation of macrophage phenotype by soluble product(s) released from neutrophils." The Journal of Immunology, vol. 174, no. 4, 2005, pp. 2265-72. |
| Daley JM, Ivanenko-Johnston T, Reichner JS, Albina JE. "Transcriptional regulation of TNF-alpha production in neutropenia." American journal of physiology. Regulatory, integrative and comparative physiology, vol. 288, no. 2, 2005, pp. R409-12. |
| Ivanov SS, Chung AS, Yuan ZL, Guan YJ, Sachs KV, Reichner JS, Chin YE. "Antibodies immobilized as arrays to profile protein post-translational modifications in mammalian cells." Mol Cell Proteomics, vol. 3, no. 8, 2004, pp. 788-95. |
| Tsikitis VL, Albina JE, Reichner JS. "Beta-glucan affects leukocyte navigation in a complex chemotactic gradient." Surgery, vol. 136, no. 2, 2004, pp. 384-9. |
| Reichner JS, Albina JE. "Determination of the role of hypoxia-inducible factor 1 in wound healing." Methods in enzymology, vol. 381, 2004, pp. 527-38. |
| Tsikitis, Vassiliki L., Martin, Anne, Albina, Jorge, Reichner, Jonathan. "Ligation of the lactosylceramide receptor (CDw17) promotes neutrophil migration." Journal of the American College of Surgeons, vol. 199, no. 3, 2004, pp. 44. |
| Tsikitis VL, Morin NA, Harrington EO, Albina JE, Reichner JS. "The lectin-like domain of complement receptor 3 protects endothelial barrier function from activated neutrophils." The Journal of Immunology, vol. 173, no. 2, 2004, pp. 1284-91. |
| Albina JE, Reichner JS. "Detection of reactive oxygen intermediate production by macrophages." Methods in molecular medicine, vol. 78, 2003, pp. 369-76. |
| Lomas JL, Chung CS, Grutkoski PS, LeBlanc BW, Lavigne L, Reichner J, Gregory SH, Doughty LA, Cioffi WG, Ayala A. "Differential effects of macrophage inflammatory chemokine-2 and keratinocyte-derived chemokine on hemorrhage-induced neutrophil priming for lung inflammation: assessment by adoptive cells transfer in mice." Shock, vol. 19, no. 4, 2003, pp. 358-65. |
| Albina JE, Reichner JS. "Oxygen and the regulation of gene expression in wounds." Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, vol. 11, no. 6, 2003, pp. 445-51. |
| Mahoney E, Reichner J, Bostom LR, Mastrofrancesco B, Henry W, Albina J. "Bacterial colonization and the expression of inducible nitric oxide synthase in murine wounds." The American Journal of Pathology, vol. 161, no. 6, 2002, pp. 2143-52. |
| Ayala A, Chung CS, Lomas JL, Song GY, Doughty LA, Gregory SH, Cioffi WG, LeBlanc BW, Reichner J, Simms HH, Grutkoski PS. "Shock-induced neutrophil mediated priming for acute lung injury in mice: divergent effects of TLR-4 and TLR-4/FasL deficiency." The American Journal of Pathology, vol. 161, no. 6, 2002, pp. 2283-94. |
| Albina JE, Mastrofrancesco B, Vessella JA, Louis CA, Henry WL Jr, Reichner JS. "HIF-1 expression in healing wounds: HIF-1alpha induction in primary inflammatory cells by TNF-alpha." AJP: Cell Physiology, vol. 281, no. 6, 2001, pp. C1971-7. |
| Harler MB, Reichner J. "Increased neutrophil motility by beta-glucan in the absence of chemoattractant." Shock, vol. 16, no. 6, 2001, pp. 419-24. |
| Reichner, Jonathan S., Fitzpatrick, Paul A., Wakshull, Eric, Albina, Jorge E. "Receptor-mediated phagocytosis of rat macrophages is regulated differentially for opsonized particles and non-opsonized particles containing beta-glucan." Immunology, vol. 104, no. 2, 2001, pp. 198-206. |
| Reichner JS, Fitzpatrick PA, Wakshull E, Albina JE. "Receptor-mediated phagocytosis of rat macrophages is regulated differentially for opsonized particles and non-opsonized particles containing beta-glucan." Immunology, vol. 104, no. 2, 2001, pp. 198-206. |
| Meszaros AJ, Reichner JS, Albina JE. "Macrophage-induced neutrophil apoptosis." The Journal of Immunology, vol. 165, no. 1, 2000, pp. 435-41. |
| Albina JE, Mastrofrancesco B, Reichner JS. "Acyl phosphatase activity of NO-inhibited glyceraldehyde-3-phosphate dehydrogenase (GAPDH): a potential mechanism for uncoupling glycolysis from ATP generation in NO-producing cells." Biochem. J., vol. 341 ( Pt 1), 1999, pp. 5-9. |
| Meszaros AJ, Reichner JS, Albina JE. "Macrophage phagocytosis of wound neutrophils." Journal of Leukocyte Biology, vol. 65, no. 1, 1999, pp. 35-42. |
| Reichner JS, Meszaros AJ, Louis CA, Henry WL Jr, Mastrofrancesco B, Martin BA, Albina JE. "Molecular and metabolic evidence for the restricted expression of inducible nitric oxide synthase in healing wounds." The American Journal of Pathology, vol. 154, no. 4, 1999, pp. 1097-104. |
| Harler MB, Wakshull E, Filardo EJ, Albina JE, Reichner JS. "Promotion of neutrophil chemotaxis through differential regulation of beta 1 and beta 2 integrins." The Journal of Immunology, vol. 162, no. 11, 1999, pp. 6792-9. |
| Louis CA, Mody V, Henry WL Jr, Reichner JS, Albina JE. "Regulation of arginase isoforms I and II by IL-4 in cultured murine peritoneal macrophages." The American journal of physiology, vol. 276, no. 1 Pt 2, 1999, pp. R237-42. |
| Hofer SO, Molema G, Hermens RA, Wanebo HJ, Reichner JS, Hoekstra HJ. "The effect of surgical wounding on tumour development." European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology, vol. 25, no. 3, 1999, pp. 231-43. |
| Nessel CC, Henry WL Jr., Mastrofrancesco B, Reichner JS, Albina JE. "Vestigial respiratory burst activity in wound macrophages." American journal of physiology. Regulatory, integrative and comparative physiology, vol. 276, no. 6, 1999, pp. R1587-R1594. |
| Nessel CC, Henry WL Jr, Mastrofrancesco B, Reichner JS, Albina JE. "Vestigial respiratory burst activity in wound macrophages." The American journal of physiology, vol. 276, no. 6 Pt 2, 1999, pp. R1587-94. |
| Louis CA, Reichner JS, Henry WL Jr, Mastrofrancesco B, Gotoh T, Mori M, Albina JE. "Distinct arginase isoforms expressed in primary and transformed macrophages: regulation by oxygen tension." The American journal of physiology, vol. 274, no. 3 Pt 2, 1998, pp. R775-82. |
| Reichner JS, Mulligan JA, Spisni R, Sotomayor EA, Albina JE, Bland KI. "Effect of IL-6 overexpression on the metastatic potential of rat hepatocellular carcinoma cells." Annals of Surgical Oncology, vol. 5, no. 3, 1998, pp. 279-86. |
| Yauch RL, Berditchevski F, Harler MB, Reichner J, Hemler ME. "Highly stoichiometric, stable, and specific association of integrin alpha3beta1 with CD151 provides a major link to phosphatidylinositol 4-kinase, and may regulate cell migration." Molecular biology of the cell, vol. 9, no. 10, 1998, pp. 2751-65. |
| Reichner JS, Helgemo SL, Hart GW. "Recycling cell surface glycoproteins undergo limited oligosaccharide reprocessing in LEC1 mutant Chinese hamster ovary cells." Glycobiology, vol. 8, no. 12, 1998, pp. 1173-82. |
| Albina JE, Reichner JS. "Role of nitric oxide in mediation of macrophage cytotoxicity and apoptosis." Cancer metastasis reviews, vol. 17, no. 1, 1998, pp. 39-53. |
| Hofer SO, Shrayer D, Reichner JS, Hoekstra HJ, Wanebo HJ. "Wound-induced tumor progression: a probable role in recurrence after tumor resection." Archives of surgery (Chicago, Ill. : 1960), vol. 133, no. 4, 1998, pp. 383-9. |
| Albina JE, Martin BA, Henry WL Jr, Louis CA, Reichner JS. "B cell lymphoma-2 transfected P815 cells resist reactive nitrogen intermediate-mediated macrophage-dependent cytotoxicity." The Journal of Immunology, vol. 157, no. 1, 1996, pp. 279-83. |
| Pricolo VE, Madhere SM, Finkelstein SD, Reichner JS. "Effects of lambda-carrageenan induced experimental enterocolitis on splenocyte function and nitric oxide production." Journal of Surgical Research, vol. 66, no. 1, 1996, pp. 6-11. |
| Reichner JS, Mulligan JA, Palla ME, Hixson DC, Albina JE, Bland KI. "Interleukin-6 production by rat hepatocellular carcinoma cells is associated with metastatic potential but not with tumorigenicity." Archives of surgery (Chicago, Ill. : 1960), vol. 131, no. 4, 1996, pp. 360-5. |
| Mateo RB, Reichner JS, Albina JE. "NO is not sufficient to explain maximal cytotoxicity of tumoricidal macrophages against an NO-sensitive cell line." Journal of Leukocyte Biology, vol. 60, no. 2, 1996, pp. 245-52. |
| Reichner JS, Mulligan JA, Bodenheimer HC Jr. "Electron transport chain activity in normal and activated rat macrophages." Journal of Surgical Research, vol. 59, no. 6, 1995, pp. 636-43. |
| Mateo RB, Reichner JS, Mastrofrancesco B, Kraft-Stolar D, Albina JE. "Impact of nitric oxide on macrophage glucose metabolism and glyceraldehyde-3-phosphate dehydrogenase activity." The American journal of physiology, vol. 268, no. 3 Pt 1, 1995, pp. C669-75. |
| Albina JE, Henry WL Jr, Mastrofrancesco B, Martin BA, Reichner JS. "Macrophage activation by culture in an anoxic environment." The Journal of Immunology, vol. 155, no. 9, 1995, pp. 4391-6. |
| Albina JE, Reichner JS. "Nitric oxide in inflammation and immunity." New horizons (Baltimore, Md.), vol. 3, no. 1, 1995, pp. 46-64. |
| Cui S, Reichner JS, Mateo RB, Albina JE. "Activated murine macrophages induce apoptosis in tumor cells through nitric oxide-dependent or -independent mechanisms." Cancer Research, vol. 54, no. 9, 1994, pp. 2462-7. |
| Coburn MC, Hixson DC, Reichner JS. "In vitro immune responsiveness of rats lacking active dipeptidylpeptidase IV." Cellular immunology, vol. 158, no. 2, 1994, pp. 269-80. |
| Mateo RB, Reichner JS, Albina JE. "Interleukin-6 activity in wounds." The American journal of physiology, vol. 266, no. 6 Pt 2, 1994, pp. R1840-4. |
| Albina JE, Cui S, Mateo RB, Reichner JS. "Nitric oxide-mediated apoptosis in murine peritoneal macrophages." The Journal of Immunology, vol. 150, no. 11, 1993, pp. 5080-5. |
| Zhou P, Reichner JS, Gorzynski, Quackenbuch LJ, Zaleski MB. "Evidence that class I-restricted response to Thy-1 antigen requires L3T4+ cells and macrophages but not Lyt-2+ cells." Transplantation, vol. 47, no. 6, 1989, pp. 1089-92. |
| Whiteheart SW, Passaniti A, Reichner JS, Holt GD, Haltiwanger RS, Hart GW. "Glycosyltransferase probes." Methods in enzymology, vol. 179, 1989, pp. 82-95. |
| Zaleski MB, Zhou P, Quackenbush LJ, Gorzynski TJ, Reichner JS. "Immunological communications." Immunologic research, vol. 8, no. 4, 1989, pp. 316-26. |
| Reichner JS, Whiteheart SW, Hart GW. "Intracellular trafficking of cell surface sialoglycoconjugates." Journal of Biological Chemistry, vol. 263, no. 31, 1988, pp. 16316-26. |
| Bayna EM, Runyan RB, Scully NF, Reichner J, Lopez LC, Shur BD. "Cell surface galactosyltransferase as a recognition molecule during development." Molecular and Cellular Biochemistry, vol. 72, no. 1-2, 1986, pp. 141-51. |
| Zaleski MB, Quackenbush LJ, Gorzynski TJ, Reichner JS. "The Ir-Thy-1 concept: continuing saga." Immunologic research, vol. 5, no. 1, 1986, pp. 79-88. |
| Zaleski MB, Reichner JS. "Class II molecules in anti-Thy-1 response of inbred mice. Studies of new Thy-1 and H-2 congenic strains." Transplantation, vol. 37, no. 1, 1984, pp. 108-10. |
| Zaleski MB, Reichner JS, Albini B. "Preliminary analysis of primary and secondary anti-Thy-1 responses elicited by immunization with cell-bound and cell-free antigen." International archives of allergy and applied immunology, vol. 73, no. 3, 1984, pp. 263-8. |
| Reichner JS, Gorzynski TJ, Zaleski MB. "New Thy-1- and H-2-congenic strains of mice and their application in studies on the mechanism of anti-Thy-1.1 response." Immunological communications, vol. 12, no. 5, 1983, pp. 501-8. |
| Zaleski MB, Reichner JS. "The Ir-Thy-1 concept: facts and interpretation." Survey of immunologic research, vol. 1, no. 1, 1982, pp. 76-84. |
| Zaleski MB, Gorzynski TJ, Reichner J. "H-2 molecules in recognition of Thy-1 antigens: facts and speculations." American journal of reproductive immunology : AJRI : official journal of the American Society for the Immunology of Reproduction and the International Coordination Committee for Immunology of Reproduction, vol. 1, no. 3, 1981, pp. 140-4. |
| Gorznski TJ, Reichner JS, Zaleski MB. "The I genotype and the anti-thy-1 response: revival of the Ir-Thy-1 concept." Transplantation, vol. 32, no. 2, 1981, pp. 158-61. |
| Reichner JS, Zaleski MB. "The search for H-2 complementation affecting the anti-Thy-1 response in mice: a progress report." Immunological communications, vol. 10, no. 6, 1981, pp. 523-31. |
Neutrophils as Tumor Killers: Neutrophils are the first line of defense against infection and exhibit a unique cell-killing mechanism called trogoptosis (“gnawing”), based on successive puncturing of cancer cell membranes. We investigate the efficacy of this mechanism in the context of different tumor cell surface markers as well as the extracellular matrix. We envision that neutrophil trogoptosis can be therapeutically enhanced for anti-tumor activity in combination with adjuvant drug therapy.
Neutrophil Mechanobiology: Inflammation is mediated by the neutrophil, a circulating white blood cell that is essential to eliminate infection, clear damaged tissue and initiate the return to health. In some diseases states inflammation becomes excessive and neutrophils cause damage to the organs they are designed to protect. Since human neutrophils cannot be studied while in the body, in vitro models are essential to understand how these cells behave. To mimic the physical nature of bodily tissues and organs, we apply different biomaterials to study neutrophils from healthy and critically ill patients as they act within the three dimensional space of an injured tissue. The long-term goal of our research is to control the activity of the cell to function optimally for the benefit of human health.
Neutrophils in the damage to vasculature endothelial cells during sepsis: Sepsis is a significant medical problem in the intensive care unit that occurs when a patient has a suspected source of infection and bodily injury which together drives the system-wide inflammatory system into hyperactivity. Neutrophils are the major inflammatory cells in the bloodstream and, when activated, can cause organ damage and can lead to death of the patient. We have identified a small fraction of all neutrophils cause most of this damage and we propose that they can be safely eliminated or blocked without causing suppression of the immune system.
Neutrophils in the Response to Fungal Pathogens: Infections with fungal pathogens can occur in patients while they are in an intensive care unit for a long period of time and are difficult to treat. Fungal pathogens are normally combated by the neutrophils in the immune system. The neutrophil response to fungi can be excessive and may cause tissue damage in excess of that needed to control the infection. This ongoing work is to test the possibility that neutrophil cell surface receptors called integrins can lessen the damaging response and maintain the protective immune response to fungi.
Neutrophils are capable of recognizing linear polymers of glucose linked in ß-1,3 conformation (viz., ß-glucan) which is a cell wall component of infectious microorganisms including yeast, fungi and bacteria. The recognition of ß-glucan by these cells is of benefit to host defense in two ways: first, since glucose does not occur in mature mammalian glycoproteins, ß-glucan provides a recognition mechanism through which neutrophils can bind and clear microorganisms without the need for opsonization; and second, ß-glucan binding stimulates neutrophil functions including chemotaxis, phagocytosis and oxidative burst. However, the mechanism(s) by which these inflammatory cells recognize ß-glucan is not defined. Work in this laboratory suggests that human neutrophils detect ß-glucan through novel interactions with cell surface integrins producing subsequent effects on cell motility. Ongoing efforts are directed at defining the mechanism(s) of this recognition and the second messenger signalling events that are triggered within the migrating cell. This work entails microscopic techniques in cell migration and immunocytochemistry, as well as protein biochemistry and molecular biology to investigate intracellular signalling pathways.
Translational Research:
2023-2025 "Investigating the ability of human blood neutrophils to kill cancer"
R03 CA280714
2019-2023 "Identification of a damaging subset of neutrophils that arises in septic patients:
RO1 HL147525
2014 –2020 “Neutrophil-Endothelial Interactions and Barrier Function in Sepsis”
R01 HL125265-01
Multi-PI: M. Kim, J. Reichner, R. Waugh
2015-2020 “Use of Biomimicry to Determine the Effect of Sepsis on Neutrophil Traction”
R01-AI116629
Multi-PI: J. Reichner and C. Franck
Johnson, C.M., O’Brien, X.M., Byrd, A.S., Parisi, V.E., Loosely, A.J., Li., W., Witt, H., Faridi, M.H., Lefort, C.T., Gupta, V., Kim, M., and Reichner, J.S. Integrin cross-talk regulates the human neutrophil response to fungal beta-glucan in the context of extracellular matrix: A prominent role for VLA3 in the anti-fungal response. J. Immunology 198:318-334, 2017
O’Brien, X.M., Biron, B.M. and Reichner, J.S. Consequences of extracellular trap formation in sepsis. Curr. Opin. Hemat., 24:66-71, 2017
Byrd, A.S., O’Brien, X.M., Laforce-Nesbitt, S.S., Parisi, V., Hirakawa, M.P., Bliss, J.M. and Reichner, J.S. NETosis in neonates: evidence of a ROS-independent pathway in response to fungal challenge. J. Infectious Diseases, 213:634-639, 2016.
Stout, D.A., Bar-Kochba, E., Estrada, J.B., Toyjanova, J., Kesari, H., Reichner, J.S. and Franck, C. Mean deformation metrics for quantifying 3D cell-matrix interactions in the absence of material properties. PNAS, 113:2898-2903, 2016. PMID: 26929377
Biron, B.M, Chung, C.S., O’Brien, X.M., Chen, Y., Reichner, J.S. and Ayala, A. Cl-amidine prevents histone 3 citrullination and neutrophil extracellular trap formation, and improves survival, in a murine sepsis model. J. Innate Immunity, (epub ahead of press), 2016.
O’Brien, X.M. and Reichner, J.S. Neutrophil integrins and matrix ligands and NET release. Front. Immunol, 7:363-366, 2016.
Toyjanova, J., Flores, E., Reichner, J.S. and Franck, C. Matrix confinement plays a pivotal role in regulating neutrophil-generated tractions, speed and integrin utilization. J. Biol. Chem. 290:3752-3763, 2015.
Loosely, A., O’Brien, X., Reichner, J.S., and Tang, J.X. Describing Directional Cell Migration with a Characteristic Directionality Time. PLOS One, 10:e0127425, 2015
Ayala, A., Elphick, G.F., Kim, Y.S., Huang, X., Carreira-Rosario, A., Santos, S.C., Shubin, N.J., Chen Y., Reichner, J.S, Chung, C.S.Sepsis-Induced Potentiation of Peritoneal Macrophage Migration Is Mitigated by Programmed Cell Death Receptor-1 Gene Deficiency. J. Innate Immunity, 6:325-338, 2014.
O’Brien, X.M., Heflin, K.E., Loosley, A., Tang, J.X. and Reichner, J.S. Introducing a Novel Metric, Directionality Time, to Quantify Human Neutrophil Chemotaxis as a Function of Matrix Composition and Stiffness. J. Leuk. Biol, 95:993-1004, 2014.
Toyjanova, J., Bar-Kochba, E., Lopez-Fagundo, C., Reichner, J.S., Hoffman-Kim, D. and Franck, C. High resolution, large deformation traction force microscopy. PLOS One, 9:e90976, 2014.
Magruder, H.T, Quinn, J.A, Schwartzbauer, J.E., Reichner, J.S, Huang, A and Filardo, E.J. The G-protein-coupled estrogen receptor, GPER-1, promotes fibrillogenesis via a Shc-dependent pathway resulting in anchorage-independent growth. Hormones and Cancer 5:390-404, 2014.
Byrd, A.S., O'Brien, X.M., Johnson, C.M., Lavigne, L.M. and Reichner, J.S. An extracellular matrix-based mechanism of rapid neutrophil extracellular trap formation in response to C. albicans. J. Immunology 190:4136-4148, 2013.
Brancato, S.K., Thomay, A.A., Daley, J.M., Crane, M.J. Reichner, J.S., Sabo, E. and Albina, J.E. Toll-like receptor 4 signaling regulates the acute local inflammatory response to injury and the fibrosis/neovascularization of sterile wounds. Wound Repair Regen. 21: 2013.
Fox, E., Heffernan, D., Cioffi, W., and Reichner, J.S. Neutrophils from critically ill patients mediate profound loss of barrier integrity. Critical Care. 17:226-232, 2013.
O'Brien XM, Heflin KE, Lavigne LM, Yu K, Kim M, Salomon AR, Reichner JS. Lectin site ligation of CR3 induces conformational changes and signaling. J Biol Chem. 287:3337-3348, 2012
Newsome CT, Flores E, Ayala A, Gregory S, Reichner J.S. Improved antimicrobial host defense in mice following poly-(1,6)-β-D-glucopyranosyl-(1,3)-β-D-glucopyranose glucan treatment by a gender-dependent immune mechanism. Clin Vaccine Immunol. 18:2043-9, 2011
Daley, J.M., Brancato, S.K., Thomay, A.A., Reichner, J.S. and Albina, J.E. The phenotype of the wound macrophage. J. Leuk. Biol. 87:1, 2010.
Oakes, P.W., Morin, N.A., Zitterbart, D.P., Patel, D. C., Fabry, B., Reichner, J.S. (Corresponding author), and Tang J.X. Neutrophil morphology and migration are affected by substrate elasticity. Blood, 114:1387-1395, 2009.
Elphick, G.F., Sarangi, P.P., Hyun, Y-M, Hollenbaugh, J.A., Ayala, A., Biffl, W.L., Chung, H-L., Rezaie, A.R., McGrath, J.L., Topham, D.J., Reichner, J.S. and Kim, M. Recombinant human activated protein C inhibits integrin-mediated neutrophil function. Blood, 113:4078-4085
Muratore, C.S., Luks, F.I., Zhou, Y., Harty, M., Reichner, J., and Tracy, T.F. Endotoxin alters early fetal lung morphogenesis. J. Surg. Res. 155:225-230, 2009.
Morin NA, Oakes PW, Hyun YM, Lee D, Chin YE, King MR, Springer TA, Shimaoka M, Tang JX, Reichner JS, Kim M. Abstract Nonmuscle myosin heavy chain IIA mediates integrin LFA-1 de-adhesion during T lymphocyte migration. J Exp Med. 2008 Jan 21;205(1):195-205.
Lavigne LM, O'Brien XM, Kim M, Janowski JW, Albina JE, Reichner JS. Integrin engagement mediates the human polymorphonuclear leukocyte response to a fungal pathogen-associated molecular pattern. J Immunol. 2007 Jun 1;178(11):7276-82.
LeBlanc, B.W., Albina, J.E. and Reichner, J.S. Effect of beta-glucan on neutrophil chemotaxis in vivo. Journal Leukocyte Biol. 79:667-675, 2006.
Lavigne LM, Albina JE, Reichner JS. beta-Glucan Is a Fungal Determinant for Adhesion-Dependent Human Neutrophil Functions. J Immunol. 177:8667-75, 2006.
Daley, J.M., Reichner, J.S., Mahoney, E.J., Manfield, L., Henry Jr., W.L., Mastrofrancesco, B., and Albina, J.E. The anti-inflammatory neutrophil: Wound neutrophils suppress macrophage TNF-alpha release. J. Immunol. 174:2265-2272, 2005.
Albina, J.E., Mahoney, E.J., Daley, J.M., Wesche, D.E, Morris, S.M., and Reichner, J.S. Macrophage arginase I regulation by C/EBPß. SHOCK 23:168-172, 2005.
Tsikitis, V., Morin, N., Harrington, E., Albina, J.E. and Reichner, J.S. Glucan protects the integrity of an endothelial monolayer in the presence of activated neutrophils. J. Immunol. 173:1284-1291, 2004.
Tsikitis, V.L., Albina, J.E., and Reichner, J.S. ß-glucan affects leukocyte navigation in complex chemotactic gradients. Surgery 136:384-389, 2004.
Ivanov, S.S., Chung, A.S., Yuan, P.Z., Guan, A.Y., Sachs, K.V., Reichner, J.S. and Chin, Y.E. Antibodies immobilized as arrays to profile protein post-translational modifications in mammalian cells. Molecular and Cellular Proteomics 3:788-795, 2004
| Year | Degree | Institution |
|---|---|---|
| 1983 | PhD | State University of New York at Buffalo |
| 1976 | BS | Ohio State University |
| Name | Title |
|---|---|
| Lefort, Craig | Assistant Professor of Surgery (Research) |
| Wong, Ian | Associate Professor of Engineering, Associate Professor of Pathology and Laboratory Medicine |
American Society of Clinical Pathologists
American Association of Immunologists
Society for Leukocyte Biology
| Professor. Surgery, 2012-2016 |
| Associate Professor. Surgery, 1997-2011 |
| Assistant Professor. Surgery, 1988-1997 |
| Research Associate. Rhode Island Hospital, 1988-Present |
| Research Associate. Johns Hopkins School of Medicine, 1985-1988 |
| Project Investigator. MD Anderson Hospital & Tumor Institute of the University of Texas Cancer Center, 1984-1985 |
| Research Assistant. University of Connecticut Health Science Center, 1983-1984 |
| Research Assistant. SUNY Buffalo, 1979-1982 |
