Postdoctoral Fellow (Sanford-Burnham Medical Research Institute)
Ph.D. Biochemistry (Dalhousie)
|Seah NE, de Magalhaes Filho CD, Petrashen AP, Henderson HR, Laguer J, Gonzalez J, Dillin A, Hansen M, Lapierre LR Autophagy-mediated longevity is modulated by lipoprotein biogenesis.. Autophagy. 2015; : 0.|
|Zhang H, Chang JT, Guo B, Hansen M, Jia K, Kovács AL, Kumsta C, Lapierre LR, Legouis R, Lin L, Lu Q, Meléndez A, O'Rourke EJ, Sato K, Sato M, Wang X, Wu F Guidelines for monitoring autophagy in Caenorhabditis elegans.. Autophagy. 2015; : 9-27.|
|Lapierre, L.R., Kumsta, C., Sandri, M., Ballabio, A. and Hansen, M. Transcriptional and epigenetic regulation of autophagy in aging. Autophagy. 2015; 11 (6) : 867-80.|
|Lapierre LR, Silvestrini MJ, Nuñez L, Ames K, Wong S, Le TT, Hansen M, Meléndez A Autophagy genes are required for normal lipid levels in C. elegans.. Autophagy. 2013; 9 (3) : 278-86.|
|Lapierre LR, De Magalhaes Filho CD, McQuary PR, Chu CC, Visvikis O, Chang JT, Gelino S, Ong B, Davis AE, Irazoqui JE, Dillin A, Hansen M The TFEB orthologue HLH-30 regulates autophagy and modulates longevity in Caenorhabditis elegans.. Nat Comms. 2013; 4 : 2267.|
|Lapierre LR, Meléndez A, Hansen M Autophagy links lipid metabolism to longevity in C. elegans.. Autophagy. 2012; 8 (1) : 144-6.|
|Lapierre LR, Hansen M Lessons from C. elegans: signaling pathways for longevity.. Trends in Endocrinology & Metabolism. 2012; 23 (12) : 637-44.|
|Lapierre LR, Gelino S, Meléndez A, Hansen M Autophagy and lipid metabolism coordinately modulate life span in germline-less C. elegans.. Current Biology. 2011; 21 (18) : 1507-14.|
|Fisher EA, Lapierre LR, Junkins RD, McLeod RS The AAA-ATPase p97 facilitates degradation of apolipoprotein B by the ubiquitin-proteasome pathway.. Journal of lipid research. 2008; 49 (10) : 2149-60.|
|Lapierre, LR, McLeod, RS Regulation of hepatic production of lipoproteins containing apolipoprotein B by ER-associated degradation. Future Lipidology. 2007; 2 (2) : 173-184.|
|Tran K, Sun F, Cui Z, Thorne-Tjomsland G, St Germain C, Lapierre LR, McLeod RS, Jamieson JC, Yao Z Attenuated secretion of very low density lipoproteins from McA-RH7777 cells treated with eicosapentaenoic acid is associated with impaired utilization of triacylglycerol synthesized via phospholipid remodeling.. Biochimica et biophysica acta. 2006; 1761 (4) : 463-73.|
|Lapierre LR, Currie DL, Yao Z, Wang J, McLeod RS Amino acid sequences within the beta1 domain of human apolipoprotein B can mediate rapid intracellular degradation.. Journal of lipid research. 2004; 45 (2) : 366-77.|
The Lapierre laboratory uses a combination of biochemical, genetic and molecular approaches to study aging in a well-established aging research model, the nematode C. elegans. Our focus is to expand our understanding of the biology of aging by investigating the autophagy/lysosomal pathway, a central longevity mechanism.
Three major projects are being pursued in our laboratory:
1. Lysosome biology: Lysosomes serve as major intracellular degradation organelles by breaking down and recycling macromolecules into metabolites that can be utilized to synthesize new molecules and organelles. In addition, lysosomes have emerged as signaling hubs that regulate how cells respond to nutrient deprivation and environmental stresses. Our goals are to understand how lysosomes modulate metabolism and signaling.
2. Regulatory network of autophagy: Autophagy, the process of engulfing its own macromolecules using lipid membranes, leads to the degradation of damaged organelles by sequestration and delivery to the lysosome. Longevity-related transcription factors mediate the activation of the transcription of numerous autophagy proteins, but their regulation is not fully understood. Our goals are to elucidate the regulatory network modulating the activity of these transcription factors as well as the epigenetic regulations associated with their targets in the context of Alzheimer’s disease and aging.
3. Lipid remodeling and signaling: Lipids have multiple roles in the cell, including membrane biology, metabolism and signaling. Lipid bilayers are essential components of organelles and the accumulation of damaged lipids is associated with accelerating aging and disease. Intracellular synthesis of lipids can drive lipid membrane renewal, but its role in aging is unclear. Our goals are to determine the contribution of lipids in organelle biogenesis and function as well as in autophagy and longevity-associated signaling.
NIA/NIH R01 AG051810
Regulation of the transcription factor HLH-30/TFEB in aging
AFAR Grant for Junior Faculty
Study of the role of lipoprotein biogenesis in autophagy regulation and lifespan determination
NIA/NIH R00 AG042494
Role of LIPL-4 in lysosomal lipolysis and aging
NIA/NIH K99 AG042494
Role of LIPL-4 in lysosomal lipolysis and aging
NIA/NIH P50 AG005131
Enhancing lysosomal lipolysis as therapy against Alzheimer’s disease (w/UCSD)
Nakamura T., Lapierre, L.R., Hansen, M. and Lipton S. (2016) The Neurobiology of aging: Free Radical Stress and Metabolic Pathways. Brocklehurst’s Textbook of Geriatric Medicine and Gerontology, 8th Edition.
National Institute on Aging (NIA) K99 Pathway to Independence Award (2013-2017)
Fishman Fund Award for Excellence in Medical Research (2013)
NIA/UCSD P50 New Investigator in Alzheimer's Disease Award (2012-2013)
BIOL 0280: Introductory Biochemistry (Metabolism section)
BIOL 2350: The Biology of Aging (Autophagy lecture)
|BIOL 0280 - Introductory Biochemistry|