We invent molecular tools to study how the brain works. Brain circuits process information in the form of electrical and chemical signals to form memories and shape behaviors. However there is no natural contrast mechanism associated with these signals. In the lab, we use bioengineering and chemical approaches to develop molecular tools to visualize and study the brain. Our research combines electrophysiology, fluorescence imaging, protein engineering, and advanced genetic approaches to visualize the structure and function of the nervous system.
Abdelfattah AS, Zheng J, Singh A, Huang YC, Reep D, Tsegaye G, Tsang A, Arthur BJ, Rehorova M, Olson CVL, Shuai Y, Zhang L, Fu TM, Milkie DE, Moya MV, Weber TD, Lemire AL, Baker CA, Falco N, Zheng Q, Grimm JB, Yip MC, Walpita D, Chase M, Campagnola L, Murphy GJ, Wong AM, Forest CR, Mertz J, Economo MN, Turner GC, Koyama M, Lin BJ, Betzig E, Novak O, Lavis LD, Svoboda K, Korff W, Chen TW, Schreiter ER, Hasseman JP, Kolb I. "Sensitivity optimization of a rhodopsin-based fluorescent voltage indicator." Neuron, vol. 111, no. 10, 2023, pp. 1547-1563.e9. |
Abdelfattah AS, Ahuja S, Akkin T, Allu SR, Brake J, Boas DA, Buckley EM, Campbell RE, Chen AI, Cheng X, Čižmár T, Costantini I, De Vittorio M, Devor A, Doran PR, El Khatib M, Emiliani V, Fomin-Thunemann N, Fainman Y, Fernandez-Alfonso T, Ferri CGL, Gilad A, Han X, Harris A, Hillman EMC, Hochgeschwender U, Holt MG, Ji N, Kılıç K, Lake EMR, Li L, Li T, Mächler P, Miller EW, Mesquita RC, Nadella KMNS, Nägerl UV, Nasu Y, Nimmerjahn A, Ondráčková P, Pavone FS, Perez Campos C, Peterka DS, Pisano F, Pisanello F, Puppo F, Sabatini BL, Sadegh S, Sakadzic S, Shoham S, Shroff SN, Silver RA, Sims RR, Smith SL, Srinivasan VJ, Thunemann M, Tian L, Tian L, Troxler T, Valera A, Vaziri A, Vinogradov SA, Vitale F, Wang LV, Uhlířová H, Xu C, Yang C, Yang MH, Yellen G, Yizhar O, Zhao Y. "Neurophotonic tools for microscopic measurements and manipulation: status report." Neurophoton, vol. 9, no. Suppl 1, 2022, pp. 013001. |
Deo, Claire, Abdelfattah, Ahmed S., Bhargava, Hersh K., Berro, Adam J., Falco, Natalie, Farrants, Helen, Moeyaert, Benjamien, Chupanova, Mariam, Lavis, Luke D., Schreiter, Eric R. "The HaloTag as a general scaffold for far-red tunable chemigenetic indicators." Nature Chemical Biology, vol. 17, no. 6, 2021, pp. 718-723. |
Abdelfattah, Ahmed S., None, None, Valenti, Rosario, Zheng, Jihong, Wong, Allan, Podgorski, Kaspar, Koyama, Minoru, Kim, Douglas S., Schreiter, Eric R. "A general approach to engineer positive-going eFRET voltage indicators." Nature Communications, vol. 11, no. 1, 2020. |
Sha F, Abdelfattah AS, Patel R, Schreiter ER. "Erasable labeling of neuronal activity using a reversible calcium marker." eLife, vol. 9, 2020. |
Qian Y, Piatkevich KD, Mc Larney B, Abdelfattah AS, Mehta S, Murdock MH, Gottschalk S, Molina RS, Zhang W, Chen Y, Wu J, Drobizhev M, Hughes TE, Zhang J, Schreiter ER, Shoham S, Razansky D, Boyden ES, Campbell RE. "A genetically encoded near-infrared fluorescent calcium ion indicator." Nature methods, vol. 16, no. 2, 2019, pp. 171-174. |
Kazemipour A, Novak O, Flickinger D, Marvin JS, Abdelfattah AS, King J, Borden PM, Kim JJ, Al-Abdullatif SH, Deal PE, Miller EW, Schreiter ER, Druckmann S, Svoboda K, Looger LL, Podgorski K. "Author Correction: Kilohertz frame-rate two-photon tomography." Nature methods, vol. 16, no. 9, 2019, pp. 932. |
Abdelfattah, Ahmed S., Kawashima, Takashi, Singh, Amrita, Novak, Ondrej, Liu, Hui, Shuai, Yichun, Huang, Yi-Chieh, Campagnola, Luke, Seeman, Stephanie C., Yu, Jianing, Zheng, Jihong, Grimm, Jonathan B., Patel, Ronak, Friedrich, Johannes, Mensh, Brett D., Paninski, Liam, Macklin, John J., Murphy, Gabe J., Podgorski, Kaspar, Lin, Bei-Jung, Chen, Tsai-Wen, Turner, Glenn C., Liu, Zhe, Koyama, Minoru, Svoboda, Karel, Ahrens, Misha B., Lavis, Luke D., Schreiter, Eric R. "Bright and photostable chemigenetic indicators for extended in vivo voltage imaging." Science, vol. 365, no. 6454, 2019, pp. 699-704. |
Shen Y, Dana H, Abdelfattah AS, Patel R, Shea J, Molina RS, Rawal B, Rancic V, Chang YF, Wu L, Chen Y, Qian Y, Wiens MD, Hambleton N, Ballanyi K, Hughes TE, Drobizhev M, Kim DS, Koyama M, Schreiter ER, Campbell RE. "Correction to: A genetically encoded Ca2+ indicator based on circularly permutated sea anemone red fluorescent protein eqFP578." BMC biology, vol. 17, no. 1, 2019, pp. 85. |
Kazemipour A, Novak O, Flickinger D, Marvin JS, Abdelfattah AS, King J, Borden PM, Kim JJ, Al-Abdullatif SH, Deal PE, Miller EW, Schreiter ER, Druckmann S, Svoboda K, Looger LL, Podgorski K. "Kilohertz frame-rate two-photon tomography." Nature methods, vol. 16, no. 8, 2019, pp. 778-786. |
Farhi SL, Parot VJ, Grama A, Yamagata M, Abdelfattah AS, Adam Y, Lou S, Kim JJ, Campbell RE, Cox DD, Cohen AE. "Wide-Area All-Optical Neurophysiology in Acute Brain Slices." Journal of Neuroscience, vol. 39, no. 25, 2019, pp. 4889-4908. |
Shen Y, Dana H, Abdelfattah AS, Patel R, Shea J, Molina RS, Rawal B, Rancic V, Chang YF, Wu L, Chen Y, Qian Y, Wiens MD, Hambleton N, Ballanyi K, Hughes TE, Drobizhev M, Kim DS, Koyama M, Schreiter ER, Campbell RE. "A genetically encoded Ca2+ indicator based on circularly permutated sea anemone red fluorescent protein eqFP578." BMC biology, vol. 16, no. 1, 2018, pp. 9. |
Wu J, Abdelfattah AS, Zhou H, Ruangkittisakul A, Qian Y, Ballanyi K, Campbell RE. "Genetically Encoded Glutamate Indicators with Altered Color and Topology." ACS Chemical Biology, vol. 13, no. 7, 2018, pp. 1832-1837. |
Abdelfattah AS, Farhi SL, Zhao Y, Brinks D, Zou P, Ruangkittisakul A, Platisa J, Pieribone VA, Ballanyi K, Cohen AE, Campbell RE. "A Bright and Fast Red Fluorescent Protein Voltage Indicator That Reports Neuronal Activity in Organotypic Brain Slices." Journal of Neuroscience, vol. 36, no. 8, 2016, pp. 2458-72. |
Abdelfattah AS, Rancic V, Rawal B, Ballanyi K, Campbell RE. "Ratiometric and photoconvertible fluorescent protein-based voltage indicator prototypes." Chem. Commun., vol. 52, no. 98, 2016, pp. 14153-14156. |
Wu J, Abdelfattah AS, Miraucourt LS, Kutsarova E, Ruangkittisakul A, Zhou H, Ballanyi K, Wicks G, Drobizhev M, Rebane A, Ruthazer ES, Campbell RE. "A long Stokes shift red fluorescent Ca2+ indicator protein for two-photon and ratiometric imaging." Nature Communications, vol. 5, 2014, pp. 5262. |
Zhao Y, Abdelfattah AS, Zhao Y, Ruangkittisakul A, Ballanyi K, Campbell RE, Harrison DJ. "Microfluidic cell sorter-aided directed evolution of a protein-based calcium ion indicator with an inverted fluorescent response." Integrative Biology, vol. 6, no. 7, 2014, pp. 714-25. |
Alford SC, Abdelfattah AS, Ding Y, Campbell RE. "A fluorogenic red fluorescent protein heterodimer." Chemistry & Biology, vol. 19, no. 3, 2012, pp. 353-60. |
Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE. "An expanded palette of genetically encoded Ca²⁺ indicators." Science, vol. 333, no. 6051, 2011, pp. 1888-91. |
Year | Degree | Institution |
---|---|---|
2016 | PhD | University of Alberta |
2009 | BS | German University in Cairo |
Postdoctoral Fellow | Howard Hughes Medical Institute, Janelia Research Campus | 2016-2021 |
2021 NIH New Innovator Award
2021 Searle Scholar Award
2021 Carney Innovation Award
2020 Burroughs Wellcome Fund Career Award at the Scientific Interface
2017 Grass Foundation Scholarship
2017 Society of General Physiology Scholar
2013 President’s Doctoral Prize of Distinction, University of Alberta
2012 Vanier Canada Graduate Scholarship
2012 Alberta Innovates Health Solutions Studentship
2010 President’s International Doctoral Award, University of Alberta
Carney Institute for Brain Science, Brown University
Visiting Scientist. Howard Hughes Medical Institute, 2021-2023 |
Robert J. and Nancy D. Carney University Assistant Professor of Brain Science. Carney Institute for Brain Science, 2021-Present |
NEUR 1510 - Neurotechnology: Molecular Tools and Methods for Neurobiology |