Office number : 401-444-1649
Dr. Brodsky's research program focuses on the regulation of metabolism in cancer and the discovery of diagnostic and prognostic tumor markers.
Dr. Brodsky received postdoctoral training from Dr. Pamela A. Silver at the Dana-Farber Cancer Institute/Harvard Medical School where he discovered mechanisms of mRNA export. He was among the first to use chromatin immunoprecipitation combined with genomic tiling arrays to define the coupling of transcription and mRNA processing.
Dr. Brodsky received his BA from the University of Pennsylvania where he worked with Dr. Ponzy Lu exploring DNA dynamics during electrophoresis. Dr. Brodsky received his PhD from MIT working with Dr. James R. Williamson where he developed a structural model of the HIV tat-TAR protein-RNA interaction using NMR spectroscopy.
Dr. Brodsky joined the Pathology department in 2014.
|Chowanadisai W, Messerli SM, Miller DH, Medina JE, Hamilton JW, Messerli MA, Brodsky AS Cisplatin Resistant Spheroids Model Clinically Relevant Survival Mechanisms in Ovarian Tumors.. PLoS ONE. 2016; 11 (3) : e0151089.|
|Wang Y, Resnick MB, Lu S, Hui Y, Brodsky AS, Yang D, Yakirevich E, Wang L Collagen type III α1 as a useful diagnostic immunohistochemical marker for fibroepithelial lesions of the breast.. Human Pathology. 2016; 57 : 176-181.|
|Wood SM, Gill AJ, Brodsky AS, Lu S, Friedman K, Karashchuk G, Lombardo K, Yang D, Resnick MB Fatty acid-binding protein 1 is preferentially lost in microsatellite instable colorectal carcinomas and is immune modulated via the interferon γ pathway.. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc. 2016;|
|Brodsky, Alexander S., Xiong, Jinjun, Yang, Dongfang, Schorl, Christoph, Fenton, Mary Anne, Graves, Theresa A., Sikov, William M., Resnick, Murray B., Wang, Yihong Identification of stromal ColXα1 and tumor-infiltrating lymphocytes as putative predictive markers of neoadjuvant therapy in estrogen receptor-positive/HER2-positive breast cancer.. BMC cancer. 2016; 16 (1) : 274.|
|Friedman K, Brodsky AS, Lu S, Wood S, Gill AJ, Lombardo K, Yang D, Resnick MB Medullary carcinoma of the colon: a distinct morphology reveals a distinctive immunoregulatory microenvironment. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc. 2016; 29 (5) : 528-41.|
|Grive KJ, Gustafson EA, Seymour KA, Baddoo M, Schorl C, Golnoski K, Rajkovic A, Brodsky AS, Freiman RN TAF4b Regulates Oocyte-Specific Genes Essential for Meiosis.. PLoS Genetics. 2016; 12 (6) : e1006128.|
|Yakirevich E, Ali SM, Mega A, McMahon C, Brodsky AS, Ross JS, Allen J, Elvin JA, Safran H, Resnick MB A Novel SDHA-deficient Renal Cell Carcinoma Revealed by Comprehensive Genomic Profiling.. The American Journal of Surgical Pathology. 2015; 39 (6) : 858-63.|
|Brown, Caitlin W., Brodsky, Alexander S., Freiman, Richard N. Abstract POSTER-BIOL-1352: Notch3 promotes anoikis resistance in ovarian cancer. Clin Cancer Res . 2015; 21 (16 Supplement) : POSTER-BIOL-1352-POSTER-BIOL-1352.|
|Brodsky AS, Fischer A, Miller DH, Vang S, MacLaughlan S, Wu HT, Yu J, Steinhoff M, Collins C, Smith PJ, Raphael BJ, Brard L Expression profiling of primary and metastatic ovarian tumors reveals differences indicative of aggressive disease.. PLoS ONE. 2014; 9 (4) : e94476.|
|Brown, C. W., Brodsky, A. S., Freiman, R. N. Notch3 Overexpression Promotes Anoikis Resistance in Epithelial Ovarian Cancer via Upregulation of COL4A2.. Molecular Cancer Research. 2014; 13 (1) : 78-85.|
|Casella, Cinzia, Miller, Daniel H., Lynch, Kerry, Brodsky, Alexander S. Oxysterols synergize with statins by inhibiting SREBP-2 in ovarian cancer cells. Gynecologic Oncology. 2014; 135 (2) : 333-341.|
|Ellermeier C, Vang S, Cleveland K, Durand W, Resnick MB, Brodsky AS Prognostic microRNA expression signature from examination of colorectal primary and metastatic tumors.. Anticancer research. 2014; 34 (8) : 3957-67.|
|Vang S, Wu HT, Fischer A, Miller DH, MacLaughlan S, Douglass E, Comisar L, Steinhoff M, Collins C, Smith PJ, Brard L, Brodsky AS Identification of ovarian cancer metastatic miRNAs.. PLoS ONE. 2013; 8 (3) : e58226.|
|Moore RG, Lange TS, Robinson K, Kim KK, Uzun A, Horan TC, Kawar N, Yano N, Chu SR, Mao Q, Brard L, DePaepe ME, Padbury JF, Arnold LA, Brodsky A, Shen TL, Singh RK Efficacy of a non-hypercalcemic vitamin-D2 derived anti-cancer agent (MT19c) and inhibition of fatty acid synthesis in an ovarian cancer xenograft model.. PLoS ONE. 2012; 7 (4) : e34443.|
|Lu S, Mukkada VA, Mangray S, Cleveland K, Shillingford N, Schorl C, Brodsky AS, Resnick MB MicroRNA profiling in mucosal biopsies of eosinophilic esophagitis patients pre and post treatment with steroids and relationship with mRNA targets.. PLoS ONE. 2012; 7 (7) : e40676.|
|Stuckey A, Fischer A, Miller DH, Hillenmeyer S, Kim KK, Ritz A, Singh RK, Raphael BJ, Brard L, Brodsky AS Integrated genomics of ovarian xenograft tumor progression and chemotherapy response.. BMC cancer. 2011; 11 : 308.|
|Miller DH, Fischer AK, Chu KF, Burr R, Hillenmeyer S, Brard L, Brodsky AS T0901317 inhibits cisplatin-induced apoptosis in ovarian cancer cells [corrected].. International journal of gynecological cancer : official journal of the International Gynecological Cancer Society. 2011; 21 (8) : 1350-6.|
|Wood JG, Hillenmeyer S, Lawrence C, Chang C, Hosier S, Lightfoot W, Mukherjee E, Jiang N, Schorl C, Brodsky AS, Neretti N, Helfand SL Chromatin remodeling in the aging genome of Drosophila.. Aging Cell. 2010; 9 (6) : 971-8.|
|Bronson MW, Hillenmeyer S, Park RW, Brodsky AS Estrogen coordinates translation and transcription, revealing a role for NRSF in human breast cancer cells.. Molecular endocrinology (Baltimore, Md.). 2010; 24 (6) : 1120-35.|
|Neretti N, Wang PY, Brodsky AS, Nyguyen HH, White KP, Rogina B, Helfand SL Long-lived Indy induces reduced mitochondrial reactive oxygen species production and oxidative damage.. Proceedings of the National Academy of Sciences. 2009; 106 (7) : 2277-82.|
|McKee AE, Neretti N, Carvalho LE, Meyer CA, Fox EA, Brodsky AS, Silver PA Exon expression profiling reveals stimulus-mediated exon use in neural cells.. Genome biology. 2007; 8 (8) : R159.|
|Carroll JS, Meyer CA, Song J, Li W, Geistlinger TR, Eeckhoute J, Brodsky AS, Keeton EK, Fertuck KC, Hall GF, Wang Q, Bekiranov S, Sementchenko V, Fox EA, Silver PA, Gingeras TR, Liu XS, Brown M Genome-wide analysis of estrogen receptor binding sites.. Nature genetics. 2006; 38 (11) : 1289-97.|
|Gama-Carvalho M, Barbosa-Morais NL, Brodsky AS, Silver PA, Carmo-Fonseca M Genome-wide identification of functionally distinct subsets of cellular mRNAs associated with two nucleocytoplasmic-shuttling mammalian splicing factors.. Genome biology. 2006; 7 (11) : R113.|
|Swinburne IA, Meyer CA, Liu XS, Silver PA, Brodsky AS Genomic localization of RNA binding proteins reveals links between pre-mRNA processing and transcription.. Genome research. 2006; 16 (7) : 912-21.|
|Carroll JS, Liu XS, Brodsky AS, Li W, Meyer CA, Szary AJ, Eeckhoute J, Shao W, Hestermann EV, Geistlinger TR, Fox EA, Silver PA, Brown M Chromosome-wide mapping of estrogen receptor binding reveals long-range regulation requiring the forkhead protein FoxA1.. Cell. 2005; 122 (1) : 33-43.|
|Brodsky AS, Meyer CA, Swinburne IA, Hall G, Keenan BJ, Liu XS, Fox EA, Silver PA Genomic mapping of RNA polymerase II reveals sites of co-transcriptional regulation in human cells.. Genome biology. 2005; 6 (8) : R64.|
|Brodsky AS, Johnston AP, Trau M, Silver PA Analysis of RNA-protein interactions by flow cytometry.. Current opinion in molecular therapeutics. 2003; 5 (3) : 235-40.|
|Brodsky AS, Silver PA A microbead-based system for identifying and characterizing RNA-protein interactions by flow cytometry.. Mol Cell Proteomics. 2002; 1 (12) : 922-9.|
|Dayie KT, Brodsky AS, Williamson JR Base flexibility in HIV-2 TAR RNA mapped by solution (15)N, (13)C NMR relaxation.. Journal of molecular biology. 2002; 317 (2) : 263-78.|
|Brodsky AS, Silver PA Identifying proteins that affect mRNA localization in living cells.. Methods. 2002; 26 (2) : 151-5.|
|Hennig M, Williamson JR, Brodsky AS, Battiste JL Recent advances in RNA structure determination by NMR.. Current protocols in nucleic acid chemistry / edited by Serge L. Beaucage ... [et al.]. 2001; Chapter 7 : Unit 7.7.|
|Brodsky AS, Silver PA Pre-mRNA processing factors are required for nuclear export.. RNA. 2000; 6 (12) : 1737-49.|
|Brodsky AS, Silver PA Nuclear transport HEATs up.. J. Geophys. Res.. 1999; 1 (3) : E66-7.|
|Brodsky AS, Erlacher HA, Williamson JR NMR evidence for a base triple in the HIV-2 TAR C-G.C+ mutant-argininamide complex.. Nucleic acids research. 1998; 26 (8) : 1991-5.|
|Brodsky AS, Williamson JR Solution structure of the HIV-2 TAR-argininamide complex.. Journal of molecular biology. 1997; 267 (3) : 624-39.|
Our goal is to understand which tumors respond to chemotherapy and which do not. We have compared primary and metastatic tumors to identify which tumors are the most aggressive and resistant to treatment. We have discovered new proteins and microRNA biomarkers strongly associated with chemotherapy response and survival. In the laboratory, we have discovered a new approach to enhance the anti-tumor activity of statins and are investigating how statins could be used to prevent tumor relapse.
My laboratory uses an variety of approaches to understand tumor relapse and chemosensitivity. We examine tumors from patients and mouse models as well as in vitro models to develop biomarkers and new drug combinations.
We have identified a key feedback loop important for the regulation of cholesterol that mediates ovarian and breast cancer growth and survival. We are investigating the mechanisms of this regulation and translating our discoveries to develop novel anti-tumor drug combinations.
We are investigating just how useful miRNA markers could be to identify tumors that do not respond to treatment. We discovered the key differences between ovarian and colorectal primary and metastatic tumors. We have discovered new microRNAs and pathways important for cancer cell survival and response to chemotherapy in ovarian and colorectal cancer. We are evaluating the function of these miRNAs and their potential role in regulating metabolism to mediate tumor growth and response to therapy.
COBRE pilot award (PI)
DEANs award (co-PI)
Mary Kay Foundation (PI) (completed)
Ellison Medical Foundation (PI) (completed)
Susan G. Komen Breast Cancer Foundation (co-PI) (completed)
ARRA Supplement: Biocurrents Research Center (PI subcontract) (completed)
DOD/CDMRP Breast Cancer Synergy Award(Completed)
NHGRI K22 Genome Scholar Career Development Award (Completed)
31. Wang, Y, Resnick, MB, Lu, S, Brodsky, AS, Yang, D, Yakirevich, Wang, L Collagen Type III alpha1 as a Useful Diagnostic Immunohistochemical Marker for Fibroepithelial Lesions of the Breast, submitted.
30. Grive, KJ, Gustafson, E, Seymour, KA, Baddoo, M, Schorl, C, Gonoski, K, Rajkovic, A, Brodsky, AS, Freiman, RN TAF4b Regulates Oocyte-Specific Genes Essential for Meiosis, in press.
29. Chowanadisai W, Messerli SM, Miller DH, Medina JE, Hamilton JW, Messerli MA, Brodsky AS*. Cisplatin Resistant Spheroids Model Clinically Relevant Survival Mechanisms in Ovarian Tumors. PloS one. 2016;11(3):e0151089. PubMed PMID: 26986722.
28. Brodsky AS*, Xiong J, Yang D, Schorl C, Fenton MA, Graves TA, Sikov WM, Resnick MB, Wang Y. Identification of stromal ColXalpha1 and tumor-infiltrating lymphocytes as putative predictive markers of neoadjuvant therapy in estrogen receptor-positive/HER2-positive breast cancer. BMC cancer. 2016;16(1):274. PubMed PMID: 27090210; PMCID: PMC4835834.
27. Friedman K, Brodsky AS, Lu S, Wood S, Gill AJ, Lombardo K, Yang D, Resnick MB. Medullary carcinoma of the colon: a distinct morphology reveals a distinctive immunoregulatory microenvironment. Mod Pathol. 2016. PubMed PMID: 26965581.
26. Yakirevich E, Ali SM, Mega A, McMahon C, Brodsky AS, Ross JS, Allen J, Elvin JA, Safran H, Resnick MB. A Novel SDHA-deficient Renal Cell Carcinoma Revealed by Comprehensive Genomic Profiling. Am. J. Surg. Pathol. 2015 Feb 25. [Epub ahead of print] (PMID: 25724004).
25. Brown CW, Brodsky AS, Freiman RN. Notch3 Overexpression Promotes Anoikis Resistance in Epithelial Ovarian Cancer via Upregulation of COL4A2. Mol Cancer Res. 2015 Jan;13(1):78-85. doi: 10.1158/1541-7786.MCR-14-0334. Epub 2014 Aug 28. (PMID: 25169943).
24. Casella C, Miller DH, Lynch K, Brodsky AS*. Oxysterols synergize with statins by inhibiting SREBP-2 in ovarian cancer cells. Gynecol Oncol. 2014 Nov;135(2):333-41. doi: 10.1016/j.ygyno.2014.08.015. Epub 2014 Aug 16. (PMID:25134999).
23. Ellermeier, C., Vang, S., Cleveland, K., Durand, W., Resnick, M. B.* and Brodsky, A. S.* (2014) “Prognostic MicroRNA Expression Signature from Examination of Colorectal Primary and Metastatic Tumors”, Anticancer Research, 34(8) 3957-67. (PMID: 25075017).
22. Brodsky, A. S.*, Fischer, A., Miller, D.H., Vang, S., MacLaughlan, S., Wu, H.-T., Yu, J., Steinhoff, M., Collins, C., Smither, J.S., and Brard, L. (2014) “Expression Profiling of Primary and Metastatic Ovarian Tumors Reveals Differences Indicative of Aggressive Disease” PLoS One, 9(4) e94476 [Epub April 14, 2014] (PMID: 24732363, PMC3986100).
21. Vang, S., Wu, H.-T., Fischer, A., Miller, D.H., MacLaughlan, S., Douglass, E., Steinhoff, M., Collins, C., Smith, P.J. Brard, L., Brodsky, A.S. (2013) “Identification of Ovarian Cancer Metastatic miRNAs” PLoS One, 8(3) e58226 [Epub March 12, 2013] (PMID: 23554878, PMCID: PMC3595263).
20. Lu, S., Vincent, M. A., Mangray, S., Cleveland, K., Shillingford, N., Schorl, C., Brodsky, A.S., Resnick, M. B. (2012) “MicroRNA Profiling in Mucosal Biopsies of Eosinophilic Esophagitis Patients Pre and Post Treatment with Steroids and Relationship with mRNA Targets” PLoS One, 7(4) e40676 [Epub July 16, 2012] (PMID: 22815788, PMCID: PMC3398046).
19. Moore, R.G., Lange, T.S., Robinson, K., Kim K.K., Uzun, A, Horan, T.C., Kawar, N., Yano, N., Chu, S.R., Mao, Q., Brard, L., DePaepe, M.E., Padbury, J.F., Arnold, L.A., Brodsky, A., Shen, T.L., Singh, R.K. (2012) “Efficacy of a non-hypercalcemic vitamin-D2 derived anti-cancer agent (MT19c) and inhibition of fatty acid synthesis in an ovarian cancer xenograft model” PLoS One; 7(4):e34443. Epub 2012 Apr 3. (PMID: 22509304, PMCID: PMC3317945.)
18. Miller D.H., Fischer A., Chu K.F., Burr R., Hillenmeyer S., Brard L., and Brodsky, A. S.* (2011) “T0901317 Inhibits Cisplatin Induced Apoptosis in Ovarian Cancer Cells” Int. J. Gynecol. Cancer [Epub Sep 5, 2011] (PMID 21921802, PMCID: PMC3203312).
17. Stuckey, A., Fischer, A., Miller, D.H., Hillenmeyer, S., Kim, K.K., Singh, R.K., Raphael, B. J., Brard, L., and Brodsky, A.S.* (2011) “Integrated Genomics of Ovarian Xenograft Tumor Progression and Chemotherapy Response”. BMC Cancer, Jul 22; 11(1): 308 [Epub]. (PMID 21781307, PMCID: PMC3155912).
16. Wood, J., Hillenmeyer, S., Lawrence, C., Chang, C., Hosier, S., Lightfoot, W., Mukerjee, E., Jiang, N., Schorl, C., Brodsky, A.S., Neretti, N., and Helfand, S. L. (2010) “Chromatin remodeling in the aging genome of Drosophila”, Aging Cell, 9(6) 971-8, Epub 2010 Oct 21 (PMID: 20961390, PMCID: PMC2980570).
15. Bronson, M. W., Hillenmeyer, S., Park, P. W., and Brodsky, A. S.* (2010) “Estrogen Coordinates Translation and Transcription Revealing a Role for NRSF in Human Breast Cancer Cells” Mol Endo, Jun;24(6):1120-35. Epub 2010 Apr 14 (PMID: 20392875, PMCID: PMC2875799).
14. Neretti, N., Wang, P. Y, Brodsky, A. S., White, K. P., Rogina, B., and Helfand, S. L. (2009) “Long-live Indy induces reduced mitochondrial ROS production and oxidative damage”, Proc Natl Acad Sci USA 106 2277 (PMID: 19164521, PMCID: PMC2629441).
13. McKee, A. M., Neretti, N., Carvalho, L. E., Meyer, C.A., Fox, E. A., Brodsky, A. S.*, and Silver, P. A. (2007) “Exon expression profiling reveals stimulus-mediated exon use in neural cells”, Genome Biology Aug 2;8(8):R159 [Epub ahead of print] (designated highly accessed). (PMID: 17683528, PMCID: PMC2374990).
12. Gama-Carvalho, M, Barbosa-Morais, N. L., Brodsky, A. S., Silver, P. and Carmo-Fonseca, M. (2006) “Genome wide identification of functionally distinct subsets of cellular mRNAs associated with two nucleocytoplasmic-shuttling mammalian splicing factors” Genome Biology Nov 30;7(11):R113 [Epub ahead of print] (designated highly accessed). (PMID: 17137510, PMCID: PMC1794580).
11. Carroll, J. S., Meyer, C. A., Song, J., Li, Wei, Geistlinger, T. R., Eeckhoute, J., Brodsky, A. S., Keeton, E.K., Fertuck, K. C., Hall, G. F., Wang, Q., Bekiranov, S., Sementchenko, V., Fox, E. A., Silver, P. A., Gingeras, T. R., Liu, X. S., Brown, M. (2006) “Genome-wide Analysis of Estrogen Receptor Binding Sites” Nature Genetics 38 1289-97 Epub 2006 Oct. (PMID: 17013392).
10. Swinburne, I, Meyer, C. A., Liu, X. S., Silver, P.A.* and Brodsky, A.S.* (2006) “Genomic Localization of RNA-binding Proteins Reveals Links Between pre-mRNA Processing and Transcription” Genome Research, 16(7), Epub Jun 12 2006. (PMID: 16769980, PMCID: PMC1484458).
9. Brodsky, A. S.*, Meyer, C. A., Hall, G., Swinburne, I., Keenan, B. K., Liu, X., Fox, E. A., and Silver, P. A. (2005) “Genomic Mapping of RNA Polymerase II Reveals Sites of Co-Transcriptional Regulation in Human Cells” Genome Biology 6(8):R64, Epub Jul 15. (PMID: 16086846, PMCID: PMC1273631) (designated highly accessed).
8. Carroll, J. S., Liu, X. S., Brodsky, A. S., Li, W., Szary, A. J., Shao, W., Meyer, C. A., Hestermann, E. V., Geistlinger, T. R., Fox, E. A., Silver, P. A., Brown, M. (2005) “Chromosome-wide Mapping of Estrogen Receptor Binding Reveals Long-range Combinatorial Regulation” Cell 122 33-43. (PMID: 16009131).
7. Brodsky AS, Silver PA. “Identifying Proteins that Affect mRNA Localization in Living Cells” (2002) Methods 26(2) 151-155. (PMID: 12054891).
6. Brodsky, A.S.*, Johnston, A.P., Trau, M., and Silver P.A. (2003) “Analysis of RNA-protein interactions by flow cytometry” Curr Opin Mol Ther. 5 235-40. (PMID: 12870432)
5. Brodsky, A. S.* and Silver, P. A. (2002) “A microbead-based system for identifying and characterizing RNA-protein interactions by flow cytometry” Mol Cell Proteomics, 1 922-929. (PMID: 12543929).
4. Dayie, K., Brodsky, A. S., and Williamson , J. R. (2002) “Base Flexibility in HIV-2 TAR RNA Mapped by Solution 15N, 13C NMR Relaxation” J. Mol. Biol., 317 263-278. (PMID: 11902842).
3. Brodsky, A. S. and Silver, P. A. (2000) “Pre-mRNA Processing Factors are Required for Nuclear Export” RNA, 6 1737 (Awarded paper of the year in RNA). (PMID: 11142374, PMCID: PMC1370044)
2. Brodsky, A. S., Erlacher, H.A., and Williamson, J.R. (1998) “NMR Evidence for a Base Triple in the HIV-2 TAR-C-G.C+ Mutant Argininamide Complex”, Nuc Acids Res, 26 1991-1995. (PMID: 9518494, PMCID: PMC147484).
1. Brodsky, A. S. and Williamson, J. R. (1997) “The Solution Structure of the HIV-2 TAR-Argininamide Complex” J. Mol. Biol. 267 634-649. (PMID: 9126842).
|1998||PhD||Massachusetts Institute of Technology|
|1993||MS||University of Pennsylvania|
|1992||BA||University of Pennsylvania|
|Freiman, Richard||Associate Professor of Medical Science|
|Raphael, Benjamin||Adjunct Associate Professor of Computer Science (Research)|
|Resnick, Murray||Professor of Pathology and Laboratory Medicine|
|Wang, Yihong||Associate Professor of Pathology and Laboratory Medicine|